柔嫩艾美耳球虫卵囊免疫后鸡盲肠扁桃体GM-CSF基因表达的动态变化

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (7): 950-954.doi:

柔嫩艾美耳球虫卵囊免疫后鸡盲肠扁桃体GM-CSF基因表达的动态变化

徐赓¹，王彩霞¹，王黎霞¹，安健¹*，李卫东²

1.北京农学院动物科学技术系，北京 102206；2.北京昌平城区动物卫生防疫站，北京 102200

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-23 发布日期:2008-07-23
通讯作者: 安健

Expressing Profiling of GM-CSF Gene in Cecal Tonsil of Chickens Vaccinated with Eimeria tenella Oocysts

XU Geng¹，WANG Cai-xia¹，WANG Li-xia¹，AN Jian¹*，LI Wei-dong²

1Faculty of Animal Science and Technology,Beijing Agricultural College,Beijing 102206,China;2Changping Animal Health and Disease Control Station,Beijing 102200,China

Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

摘要/Abstract

摘要： 为检测和评价鸡粒细胞巨噬细胞集落刺激因子（ChGM-CSF）在抗球虫免疫过程中的作用，以RT-PCR技术从鸡盲肠扁桃体中克隆获得了ChGM-CSF编码基因的开放阅读框（ORF），其长为435 bp。将试验鸡随机分为对照组和试验组，试验组于12、22日龄时用E. tenella孢子化卵囊进行免疫，分别于设定时间每次取5只鸡采集盲肠扁桃体，以鸡β-actin为参照建立半定量RT-PCR方法检测ChGM-CSF的表达动态。结果表明，在第1次免疫后的第1天（13日龄，P＜0.05）和第2次免疫后的第7天（29日龄，P＜0.01），试验组ChGM-CSF的表达量显著高于对照组；在第1次免疫后的第9天（21日龄）和第2次免疫后的第3天（25日龄）ChGM-CSF的表达量显著低于对照组（P＜0.01）。本研究提示ChGM-CSF参与了鸡体的抗球虫感染过程，为进一步探讨ChGM-CSF的生物学活性及作为鸡球虫病基因工程疫苗佐剂的研究奠定基础。

Abstract: In order to evaluate the effects of chicken granulocyte-macrophage colony stimulating factor(GM-CSF) on the anticoccidial immunity, the ORF of ChGM-CSF were cloned from chicken cecal tonsil using RT-PCR with a pair of specific primers designed from the reported sequence in GenBank. The full-length of ChGM-CSF ORF is 435 bp.The semi-quantitative RT-PCR using chicken β-actin as inner-reference for detecting the expression of ChGM-CSF was further developed and then was used for detecting the expressing patterns of ChGM-CSF in chickens vaccinated with sporulated oocysts of E.tenella at 12 and 22 day-old.Our results indicated that the expression level of ChGM-CSF in experimental group significantly increased at day 1 post-primary(P＜0.05) and day 7 post-boost(P＜0.01) infection,decreased significantly(P＜0.01)at day 9 post-primary and at day 3 post-boost infection.It is suggested that ChGM-CSF can play important roles in the immune responses against E.tenella infection in chickens.

徐赓;王彩霞;王黎霞;安健;李卫东. 柔嫩艾美耳球虫卵囊免疫后鸡盲肠扁桃体GM-CSF基因表达的动态变化[J]. 畜牧兽医学报, 2008, 39(7): 950-954.

XU Geng;WANG Cai-xia;WANG Li-xia;AN Jian;LI Wei-dong. Expressing Profiling of GM-CSF Gene in Cecal Tonsil of Chickens Vaccinated with Eimeria tenella Oocysts[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(7): 950-954.

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