猪带绦虫六钩蚴TSOL18基因疫苗表达载体的构建及其免疫原性研究

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (7): 945-949.doi:

猪带绦虫六钩蚴TSOL18基因疫苗表达载体的构建及其免疫原性研究

郭爱疆^1,2，才学鹏²*，房永祥²，贾万忠²，骆学农²，刘红霞²

1. 甘肃农业大学动物医学院，兰州 730070； 2. 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室，兰州 730046

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-23 发布日期:2008-07-23
通讯作者: 才学鹏

Construction of Gene Vaccine Vector Carrying TSOL18 Gene of Taenia solium Oncosphere and Its Immunogenicity

GUO Ai-jiang^1,2, CAI Xue-peng²*, FANG Yong-xiang², JIA Wan-zhong², LUO Xue-nong², LIU Hong-xia²

1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;2. Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-07-23 Published:2008-07-23

摘要/Abstract

摘要： 为研制新型猪囊尾蚴疫苗，将猪带绦虫六钩蚴阶段编码 TSOL18 的基因定向克隆于真核表达质粒 pVAX1，经筛选、鉴定及 DNA序列分析正确后，将重组质粒 pVAX1/TSOL18 转染 BHK-21 细胞，通过 SDS-PAGE、Western blotting、免疫荧光试验等方法检测细胞中表达的 TSOL18 抗原。结果表明，重组真核质粒 pVAX1/TSOL18 可在 BHK-21 细胞中表达TSOL18 目的蛋白，表达蛋白能被猪囊尾蚴病阳性血清所识别。动物免疫试验表明，真核表达载体能有效地诱导机体产生细胞免疫和体液免疫应答，这为猪囊尾蚴病基因疫苗的研究奠定了良好基础。

Abstract: In this study, recombinant expression plasmid pVAX1/TSOL18 was constructed and then transfected into BHK-21 cell line with liposome 2000. The results of SDS-PAGE, Western blotting and fluorescence antibody assay showed that TSOL18 protein was successfully expressed in BHK-21 cell line and could be recognized by positive serum against cysticercosis.Immunization in mice indicated that the expression vectors could efficiently induce the specific cellular and humoral immunoresponse, which laid a solid foundation for the further development of new vaccines of cysticercosis.

郭爱疆;才学鹏;房永祥;贾万忠;骆学农;刘红霞. 猪带绦虫六钩蚴TSOL18基因疫苗表达载体的构建及其免疫原性研究[J]. 畜牧兽医学报, 2008, 39(7): 945-949.

GUO Ai-jiang;CAI Xue-peng;FANG Yong-xiang;JIA Wan-zhong;LUO Xue-nong;LIU Hong-xia. Construction of Gene Vaccine Vector Carrying TSOL18 Gene of Taenia solium Oncosphere and Its Immunogenicity[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(7): 945-949.

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