Abstract: The development potentiality of the matured Newzeland rabbit oocytes that were activated by different ways and cultured in different media was compared. The result is that the electric pulse or chemical activation could not activate the oocytes perfectly when they were used separately, but the combination of electric pulse and chemical activation could activate the oocytes more perfectly. B2 medium is better than other media to support the development of the parthenogenetic rabbit embryos by comparison. The study offered an optimized system for the rabbit oocyte activation and cultures same as fertilization in vivo, which will supply good condition for rabbit nuclear transfer.