中国荷斯坦牛DGAT1基因与产奶性状关联分析

畜牧兽医学报 ›› 2008, Vol. 39 ›› Issue (12): 1661-1664.doi:

中国荷斯坦牛DGAT1基因与产奶性状关联分析

贾晋^1，2，马妍¹，孙东晓¹，张毅¹，张沅¹*

1.中国农业大学动物科技学院，北京 100193；2.内蒙古师范大学附属中学，呼和浩特 010020

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-12-24 发布日期:2008-12-24
通讯作者: 张沅

Association Analysis between DGAT1 Gene and Milk Production Traits in Chinese Holstein

JIA Jin^1，2，MA Yan¹，SUN Dong-xiao¹，ZHANG Yi¹，ZHANG Yuan¹*

1.College of Animal Science and Technology, China Agricultural University, Beijing 100193, China；2.Affiliated High School of InnerMongolian Normal University, Hohhot 010020, China

Received:1900-01-01 Revised:1900-01-01 Online:2008-12-24 Published:2008-12-24

摘要/Abstract

摘要： 以北京地区来自17个公牛家系的1 222头中国荷斯坦母牛为试验材料，采用PCR-RFLP技术对DGAT1基因进行了遗传多态性分析，共检测到KK、KA和AA 3种基因型，频率分别为0.143 2、0.609 7和0.247 1；采用混合动物模型对数据进行拟合，通过SAS（8.02）软件对5个产奶性状与DGAT1基因的关联程度进行了统计分析。结果表明，DGAT1不同基因型间的305 d产奶量、乳脂量、乳蛋白量差异极显著（P<0.01）；多重比较结果显示：AA基因型的305 d产奶量和乳蛋白量显著高于KK基因型（P<0.01），其乳脂量显著低于KK基因型（P<0.01）。结果提示DGAT1基因对奶牛产奶性状具有较大遗传效应，可以用于中国荷斯坦牛产奶性状的分子标记辅助选择。

Abstract: With PCR-RFLP technique, the polymorphism in the exon8 of DGAT1 gene were investigated in a population of 1 222 Chinese Holstein cows from 17 bull families. Three kinds of genotypes were found and the genotypic frequencies of KK, KA and AA were 0.143 2, 0.609 7 and 0.247 1, respectively-Using SAS (8.02) software with animal model, significant associations between the K232A mutation within exon8 of DGAT1 gene and three milk production traits were revealed. Duncan’s multiple-range test showed that the cows with AA genotype had higher 305day milk yield and protein yield than those with KA and KK genotypes (P<0.01), while had lower fat yield (P<0.01). The results suggested that DGAT1 gene has a great effect on milk production traits and could be a useful genetic marker in Chinese Holstein breeding.

贾晋;马妍;孙东晓;张毅;张沅. 中国荷斯坦牛DGAT1基因与产奶性状关联分析[J]. 畜牧兽医学报, 2008, 39(12): 1661-1664.

JIA Jin;MA Yan;SUN Dong-xiao;ZHANG Yi;ZHANG Yuan. Association Analysis between DGAT1 Gene and Milk Production Traits in Chinese Holstein[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2008, 39(12): 1661-1664.

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