猪传染性胃肠炎病毒SCY株3′端8.5 kb片段的克隆及序列特征分析

畜牧兽医学报 ›› 2007, Vol. 38 ›› Issue (1): 66-71.doi:

猪传染性胃肠炎病毒SCY株3′端8.5 kb片段的克隆及序列特征分析

殷华平;郭万柱;宋振辉;孙志勇;徐志文;王小玉;陈进会;杨丽

1四川农业大学动物生物技术中心，雅安 625014；2广东东莞市出入境检验检疫局，东莞 523072；3 四川农业大学动物科技学院，雅安 625014

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-01-25 发布日期:2007-01-25

Cloning and Analysis of a 8.5 kb Sequence from the 3′ End of Transmissible Gastroenteritis Virus Genome

1Animal Biotechnology Centre, Sichuan Agricultural University ,Yaan 625014,China;2Dongguan EntryExit Inspection And Quarantine Bureau,Dongguan 523072,China; 3 Animal Science and Techology College, Sichuan Agricultural University,Yaan 625014,China

Received:1900-01-01 Revised:1900-01-01 Online:2007-01-25 Published:2007-01-25

摘要/Abstract

摘要： 根据GenBank的核酸序列，设计6对引物自TGEV SCY株中分别扩增了长为1.5 kb、2.0 kb、1.1 kb、1.4 kb、1.4 kb、1.3 kb的6个片段，经克隆测序拼接后获得8 495 bp的TGEV部分序列。根据与其他TGEV和冠状病毒的序列和结构特征比较表明，该段序列克隆了除TGEV聚合酶基因以外的S、M、N、E、NSP3a、NSP3b、ORF7共7个基因的核酸序列；根据S、M、N基因的序列构建了系统进化树，分析表明TGEV-SCY株与TS、HN2002、TO14、pudue等毒株亲缘关系较近；密码子偏爱性分析结果认为TGEV在对密码子的使用上存在轻微的偏向性，个别氨基酸偏向于选择以A和T结尾的密码子。

Abstract: Six fragments about 1.5 kb,2.0 kb,1.1 kb,1.4 kb,1.4 kb,1.3 kb of transmissible gastroenteritis virus strain SCY were amplified by PCR , then were cloned into the pGMT vector and sequences were determined in Takara . 8 495 bp sequence was obtained after assembling by CAP program.Bioinformatics analysis showed that the 8 495 bp sequence included S,M,N,E,NSP3a,NSP3b,ORF7 genes. Phylogenetic trees based on S,M,N genes showed that TGEV SCY strain was closed to the TS,HN2002,TO14,pudue strains.TGEV SCY didn’t bias remarkably on any codons,but some of the amino acids biased slightly on the codons which ended by A or T.

殷华平;郭万柱;宋振辉;孙志勇;徐志文;王小玉;陈进会;杨丽. 猪传染性胃肠炎病毒SCY株3′端8.5 kb片段的克隆及序列特征分析[J]. 畜牧兽医学报, 2007, 38(1): 66-71.

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