Abstract: A 4×2(Cu×VA) experiment with repeats was designed to investigate the influence of various dietary Cu and vitamin A levels on performance, immunology function, serum CuZn-SOD activity and insulin concentration in broilers.Broilers were randomly allotted to eight groups, each of which was replicated four times with four teen broilers per replicate.14 for each treatment. Results showed that the Cu requirement was different in different stages. From 0 to 4 weeks, dietary Cu supplemental level was 8 mg/kg diet , and from 5 to 7weeks was 0-8 mg/kg diet. Dietary high copper levels(150 and 225 mg/kg diet) suppressed performance , immune function , CuZn-SOD activation and insulin concentration significantly through the whole period. When vitamin A supplemental level was 5000 IU/kg diet, better performance and other indexes was obtained through the whole period, but high vitamin A significantly(P<0.05) suppressed serum insulin concentration .CuVA interaction significantly(P<0.05) affected body weight gain at the end of week 4, feed∶ gain ratio through the whole stage，ANAE+% of lymphocyte at the end of week 7 , serum CuZn-SOD and insulin concentration at the end of week 4（P<0.01）.