Abstract: Genetic variation of the 5′ region of the IGF-Ⅰgene in 200 Shouguang chickens were detected by PCR-RELP. Three genotypes AA，AB and BB were identified after the PCR products were cut by PstⅠrestriction enzyme and their frequencies were 0.335，0.485 and 0.180, respectively. They were in Hardy-Weinberg equilibrium at the gene locus by χ² test（P＞0.05）. The associations were observed between genotypes and carcass traits including semieviscerated weight and breast muscle weight (P＜0.05). The results of the least squares means showed that a significant different effect between genotype BB and AB or AA was found in semieviscerated weight of Shouguang chickens (P＜0.05), while the significant difference was found only in breast muscle weight of the male chickens and only in liver weight of female chickens (P＜0.05). In addition, genotype BB tended to express a higher body weight in 10 weeks, semi-eviscerated weight and breast muscle weight than the other genotypes in Shouguang chickens. These results may be applied to marker assisted selection.