我国主要地方绵羊品种mtDNA细胞色素b基因PCR-RFLP研究

畜牧兽医学报 ›› 2005, Vol. 36 ›› Issue (7): 649-653.doi:

我国主要地方绵羊品种mtDNA细胞色素b基因PCR-RFLP研究

巩元芳;李祥龙;刘铮铸

1.河北科技师范学院动物科学系，昌黎 066600； 2. 河北农业大学动物科技学院，保定 071001

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2005-07-25 发布日期:2005-07-25

Study on mtDNA Cyt b Gene of Chinese Main Indigenous Sheep Breeds Using PCR-RFLP

GONG Yuan-fang;LI Xiang-long;LIU Zheng-zhu

1.Department of Animal Science, Hebei Normal University of Science & Technology, Changli 066600,China；2. College of Animal Science and Technology, Hebei Agricultural University, Baoding 071001,China

Received:1900-01-01 Revised:1900-01-01 Online:2005-07-25 Published:2005-07-25

摘要/Abstract

摘要： 应用PCR-RFLP方法，对我国9个地方绵羊品种和3个引入品种共计120个个体的线粒体DNA(mtDNA)细胞色素b(cyt b)基因进行了分析。结果表明，12种限制性内切酶在所研究的个体中共检测到22个酶切位点，15种限制性态型，其中StuⅠ、EcoRⅠ和MboⅡ表现出多态，15种限制性态型归结为4种基因单倍型，即单倍型Ⅰ、单倍型Ⅱ、单倍型Ⅲ和单倍型Ⅳ。单倍型Ⅰ为受试绵羊品种的基本单倍型。线粒体DNA细胞色素b基因遗传多态度为0.014 2%，说明我国地方绵羊品种线粒体DNA多态度贫乏。

Abstract: Technique of polymerase chain reaction(PCR) and method of restriction fragment length polymorphism(RFLP) were performed to analyse Cytochrome b(Cyt b) gene of mtDNA in 9 indigenous sheep breeds and 3 imported sheep breeds.The results indicated that 22 restriction sites and 15 restriction morphs were detected with the digestion of 6 endonucleases in all individuals,and StuⅠ, EcoRⅠ and MboⅡ were polymorphic.Fifteen restriction morphs were sorted into 4 haplotypes.The haplotype I was the main haplotype.The averaged polymorphic degree(π value=0.014 2%) of mtDNA Cyt b gene showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor.

巩元芳;李祥龙;刘铮铸. 我国主要地方绵羊品种mtDNA细胞色素b基因PCR-RFLP研究[J]. 畜牧兽医学报, 2005, 36(7): 649-653.

GONG Yuan-fang;LI Xiang-long;LIU Zheng-zhu. Study on mtDNA Cyt b Gene of Chinese Main Indigenous Sheep Breeds Using PCR-RFLP[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2005, 36(7): 649-653.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.xmsyxb.com/CN/

https://www.xmsyxb.com/CN/Y2005/V36/I7/649