鹿茸生长期鹿血多肽对小鼠雄激素性脱发的治疗作用及机制研究

doi:10.11843/j.issn.0366-6964.2026.01.038

畜牧兽医学报 ›› 2026, Vol. 57 ›› Issue (1): 432-442.doi: 10.11843/j.issn.0366-6964.2026.01.038

鹿茸生长期鹿血多肽对小鼠雄激素性脱发的治疗作用及机制研究

高琳琳¹^,²(), 管雨欣¹^,², 李吉萍², 汪珍¹^,², 任晶², 郭倩倩¹^,²(), 李春义¹^,²()

^1.吉林农业大学生命科学学院，长春 130118
^2.长春科技学院鹿茸科学与产品技术研究所，长春 130600

收稿日期:2025-04-07 出版日期:2026-01-23 发布日期:2026-01-26
通讯作者: 郭倩倩,李春义 E-mail:2863540824@qq.com;gqqice@163.com;lichunyi1959@ 163.com
作者简介:高琳琳，硕士，主要从事鹿血的相关研究，E-mail：2863540824@qq.com
基金资助:
吉林省自然科学基金项目(YDZJ202201ZYTS463);国家自然科学基金面上项目(32470892);吉林省发展和改革委员会项目(2023C040-9)

Therapeutic Efficacy of Deer Blood Polypeptides Collected during Antler Regeneration Period in Androgenetic Alopecia in Mice

GAO Linlin¹^,²(), GUAN Yuxin¹^,², LI Jiping², WANG Zhen¹^,², REN Jing², GUO Qianqian¹^,²(), LI Chunyi¹^,²()

^1.College of Life Sciences，Jilin Agricultural University，Changchun 130118，China
^2.Institute of Antler Science and Product Technology，Changchun University of Science and Technology，Changchun 130600，China

Received:2025-04-07 Online:2026-01-23 Published:2026-01-26
Contact: GUO Qianqian, LI Chunyi E-mail:2863540824@qq.com;gqqice@163.com;lichunyi1959@ 163.com

摘要/Abstract

摘要：

本研究旨在探讨鹿茸生长期鹿血多肽（ARPBP）对雄激素性脱发的治疗作用及机制。通过利用ARPBP治疗睾酮（TES）诱导的C57BL小鼠脱发模型，对其背部皮肤组织进行HE染色、免疫组化染色、qPCR与Western blot试验。结果显示：其能显著促进毛发再生，并且疗效存在浓度依赖性效应。组织学分析表明，TES模型组皮肤厚度、毛囊密度及直径显著降低，符合雄激素性脱发的病理特征。ARPBP干预后，15 mg·mL^-1低剂量ARPBP治疗组皮肤厚度及毛囊形态参数恢复接近生理水平，并且其毛发密度及覆盖率优于75 mg·mL^-1高剂量治疗组，表明15 mg·mL^-1 ARPBP组能更有效逆转睾酮诱导的毛囊微型化。进一步免疫组化结果显示，15 mg·mL^-1 ARPBP显著上调毛囊增殖标志物KI67和PCNA的表达，并增强Wnt/β-catenin通路上游因子LEF的核定位信号。Western blot分析进一步证实，ARPBP显著逆转雄激素对LEF1蛋白表达的抑制作用，并抑制雄性激素对BMP4信号通路的激活。本研究用小鼠模型揭示了ARPBP通过剂量依赖性有效治疗雄激素脱发的作用，其机制可能是通过激活LEF1-Wnt/β-catenin信号通路、抑制BMP信号通路促进雄激素诱导下毛囊由休止期进入生长期，从而促进毛发再生，这为开发基于天然多肽的精准医学策略奠定了实用化的基础。

关键词: 鹿血多肽（ARPBP）, 雄激素性脱发, 再生

Abstract:

The purpose of this study was to investigate the therapeutic effects of deer blood polypeptide （ARPBP） in the treatment of androgenic alopecia and to reveal underlying. By using ARPBP to treat testosterone （TES）-induced C57BL mouse alopecia model， HE staining， immunohistochemical staining qPCR and Western blot experiments were performed on the skin tissue of its back. Results showed that it significantly promoted hair regeneration， and the efficacy exhibited a concentration-dependent manner. Histological analysis showed that the skin thickness， follicle density and diameter in TES group were significantly reduced， which was consistent with the pathological characteristics of androgenic alopecia. After the treatment with ARPBP， the skin thickness and parameters of hair follicle shape in the low-dose ARPBP treatment group （15 mg·mL^-1） recovered nearly to the physiological level， and the hair density and area of hair coverage were better than those of the high-dose treatment group （75 mg·mL^-1）， indicating that the low-dose ARPBP group could reverse the testosterone induced hair follicle miniaturization more effectively. Immunohistochemical results showed that low-dose （15 mg·mL^-1） ARPBP significantly up-regulated the expression of follicle proliferation markers KI67 and PCNA， and enhanced the nuclear localization signal of upstream factor LEF in Wnt/β-catenin pathway. Western blot analysis further confirmed that low-dose ARPBP significantly reversed the inhibitory effect of androgen on LEF1 protein expression， and inhibited the activation of androgen on BMP4 signaling pathway. This study revealed the effective dose-dependent effect of ARPBP in the treatment of androgen alopecia. The mechanism underlying these achieved results could be via promoting the transition of androgen-induced hair follicles from resting period to growth period by activating LEF1-Wnt/β-catenin signaling pathway and inhibiting BMP signaling pathway， thus promoting hair regeneration. We believe our study has laid the foundation for the development of precision medicine strategies based on natural peptides.

Key words: deer blood polypeptide （ARPBP）, androgenic alopecia, regeneration

中图分类号:

S865.42

高琳琳, 管雨欣, 李吉萍, 汪珍, 任晶, 郭倩倩, 李春义. 鹿茸生长期鹿血多肽对小鼠雄激素性脱发的治疗作用及机制研究[J]. 畜牧兽医学报, 2026, 57(1): 432-442.

GAO Linlin, GUAN Yuxin, LI Jiping, WANG Zhen, REN Jing, GUO Qianqian, LI Chunyi. Therapeutic Efficacy of Deer Blood Polypeptides Collected during Antler Regeneration Period in Androgenetic Alopecia in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 432-442.

图/表 6

表1

图1

图2

图3

图4

图5

参考文献 21

[1]	HANS J，DAVID H，ASHLEY K C，et al.Effectiveness of combined oral minoxidil and finasteride in male androgenetic alopecia：A retrospective service evaluation［J］.Cureus，2025，17 （1）：e77549.
[2]	WANG T L，ZHOU C，SHEN Y W，et al.Prevalence of androgenetic alopecia in China：a community-based study in six cities［J］.Br J Dermatol，2010，162（4）：843-847.
[3]	WILLIAMSON D，GONZALEZ M，FINLAY A Y.The effect of hair loss on quality of life［J］.J Eur Acad Dermatol Venereol，2001，15（2）：137-139.
[4]	LOLLI F，PALLOTTI F，ROSSI A，et al.Androgenetic alopecia：a review［J］.Endocrine，2017，57（1）：9-17.
[5]	ZHOU L，WANG H，JING J，et al.Regulation of hair follicle development by exosomes derived from dermal papilla cells［J］.Biochem Biophys Res Commun，2018，500（2）：325-332.
[6]	GUPTA A K，TALUKDER M，WILLIAMS G.Comparison of oral minoxidil，finasteride，and dutasteride for treating androgenetic alopecia［J］.J Dermatolog Treat，2022，33（7）：2946-2962.
[7]	YAN H L，GAO Y，DING Q，et al.Exosomal micro rnas derived from dermal papilla cells mediate hair follicle stem cell proliferation and differentiation［J］.Int J Biol Sci，2019，15（7）：1368-1382.
[8]	GUO Q Q，LUI Z，ZHEN J J，et al.Substances for regenerative wound healing during antler renewal stimulated scar-less restoration of rat cutaneous wounds［J］.Cell Tissue Res，2021，386（1）：99-116.
[9]	GUO Q Q，ZHANG G K，REN J，et al.Systemic factors associated with antler growth promote complete wound healing［J］.NPJ Regenerat Med，2025，10（1）：4.
[10]	MARKS H D，SENNA M M.Androgenetic alopecia in gender minority patients［J］.Dermatol Clin，2020，38（2）：239-247.
[11]	寇泽.雄激素性脱发影响因素分析及预测模型构建［D］.桂林：桂林医学院，2024.
	KOU Z.Analysis of factors influencing anddrogeneticalopecia construction of prediction［D］.Guilin：Guilin Medical University，2024.（in Chinese）
[12]	丁娟，谢君，宋继权.影响男性雄激素性脱发严重程度的相关因素及其皮肤镜特征分析［J］.武汉大学学报（医学版），2023，44（1）：75-79.
	DING J，XIE J，SONG J Q.Related factors affecting the severity of male androgenetic alopecia and the dermatoscopic features analysis［J］.Medical Journal of Wuhan University，2023，44（1）：75-79.
[13]	AREEJ A.The effectiveness of treatments for androgenetic alopecia：A Systematic review and meta-analysis［J］.J Am Acad Dermatol，2017，77（1）：136-141.e5.
[14]	KEVIN M E，JOHN P S.Innovative strategies for the discovery of new drugs against androgenetic alopecia［J］.Exp Opin Drug Discovery，2025，20（4）：517-536.
[15]	ZHU N X，LIN E，ZHANG H，et al.LncRNA H19 overexpression activates Wnt signaling to maintain the hair follicle regeneration potential of dermal papilla cells［J］.Front Genet，2020，11：694.
[16]	ANJUM M A，ZULFIQAR S，CHAUDHARY A A，et al.Stimulation of hair regrowth in an animal model of androgenic alopecia using 2-deoxy-D-ribose［J］.Front Pharmacol，2024，15：1370833.
[17]	CHANG B，HWANG Y，KIM I，et al.Moracin M promotes hair regeneration through activation of the WNT/β-catenin pathway and angiogenesis［J］.Arch Dermatol Res，2025，317（1）：304.
[18]	ANDL T，REDDY T S，GADDAPARA T，et al.WNT signals are required for the initiation of hair follicle development［J］.Dev Cell，2002，2（5）：643-653.
[19]	KINDE Z M，MEKURIA A T，GESSESE T A，et al.Molecular mechanisms of hair follicle development［J］.The Scientific World Journal，2024，2024：5259055.
[20]	LIU M H，HE G Y，WANG F L，et al.Pilose antler extract promotes hair growth in androgenic alopecia mice by promoting the initial anagen phase［J］.Biomed Pharmacother，2024，174：116503.
[21]	HE X M，QI Z L，TENG R，et al.Characterization of growth secondary hair in Min pig activated by follicle stem cell stimulated by Wnt and BMP signaling pathway［J］.Animals （Basel），2023，13（7）：1239.

目的基因 Target gene	基因号 Gene number	引物序列（5’→3’） Primer sequence	目的片段大小/bp Target fragment size
Wnt10b	22 410	F：GGGTCTCCTGTTCTTGGCTT R：GCTGTCGCTTACTCAGTCCG	131
LEF1	16 842	F：TGTTTATCCCATCACGGGTGG R：CATGGAAGTGTCGCCTGACAG	189
β-catenin	12 387	F：CTCTCCACATCCTTGCTCGG R：GTGGCTCCCTCAGCTTCAAT	148
GAPDH	14 433	F：GGAGAGTGTTTCCTCGTCCC R：GATGGGCTTCCCGTTGATGA	228

鹿茸生长期鹿血多肽对小鼠雄激素性脱发的治疗作用及机制研究

Therapeutic Efficacy of Deer Blood Polypeptides Collected during Antler Regeneration Period in Androgenetic Alopecia in Mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 21

相关文章 4

编辑推荐

Metrics

本文评价

[1]	薛霖莉, 孙睿, 郝晓静, 曹校瑞, 王海东, 卢嘉茵. 基于小鼠骨骼肌损伤模型分析丹参素对骨骼肌损伤后修复再生的促进作用[J]. 畜牧兽医学报, 2023, 54(12): 5252-5263.
[2]	李凡, 陈晓阳, 杨国雨, 李福昌. Wnt/β-连环蛋白信号通路在哺乳动物毛囊发育中的调控作用[J]. 畜牧兽医学报, 2019, 50(11): 2186-2194.
[3]	董振，王权威，刘振，孙红梅，李春义. 梅花鹿致敏与休眠鹿茸干细胞差异蛋白表达的2D-DIGE分析[J]. 畜牧兽医学报, 2016, 47(1): 91-104.
[4]	陈兴勇，谢珊珊，周丽，姜润深，耿照玉. 皖西白鹅换羽前后基因表达谱差异分析[J]. 畜牧兽医学报, 2013, 44(7): 1030-1036.