表达猫杯状病毒NS2质粒转染猫肾传代细胞的转录组学分析

doi:10.11843/j.issn.0366-6964.2025.12.036

畜牧兽医学报 ›› 2025, Vol. 56 ›› Issue (12): 6339-6350.doi: 10.11843/j.issn.0366-6964.2025.12.036

表达猫杯状病毒NS2质粒转染猫肾传代细胞的转录组学分析

陈尚琛^1,2, 董宁宁^1,2, 谈晓梅², 李娜², 张琦², 罗茂青², 邓应坤², 庞帅赛², 高嘉溪², 刘光清^2*, 孟春春^2*

1. 新疆农业大学动物医学学院, 乌鲁木齐 830052;
2. 中国农业科学院上海兽医研究所伴侣动物生物安全风险预警与防控技术团队, 上海 200241

收稿日期:2025-02-26 发布日期:2025-12-24
通讯作者: 孟春春,主要从事猫病毒性传染病的致病机制研究,E-mail:mengcc@shvri.ac.cn;刘光清,主要从事病毒学基础研究,E-mail:liugq@shvri.ac.cn
作者简介:陈尚琛(1999-)，男，江苏盐城人，硕士生，主要从事猫病毒性传染病的致病机制研究，E-mail:chenshangchen17@gmail,com;董宁宁(1996-)，女，河南商丘人，硕士，主要从事猫病毒性传染病的致病机制研究，E-mail:a12367543@outlook.com
基金资助:
国家自然基金面上项目(32272982)；上海市自然基金项目(23ZR1477100)

Differential Transcriptomic Analysis of Feline Kidney Cells Transfected with Feline Calicivirus NS2 Protein

CHEN Shangchen^1,2, DONG Ningning^1,2, TAN Xiaomei², LI Na², ZHANG Qi², LUO Maoqing², DENG Yingkun², PANG Shuaisai², GAO Jiaxi², LIU Guangqing^2*, MENG Chunchun^2*

1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China;
2. Companion Animal Biosafety Risk Warning and Control Technology Team, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China

Received:2025-02-26 Published:2025-12-24

摘要/Abstract

摘要： 旨在研究FCV NS2蛋白与宿主细胞之间的相互关系。本研究用FCV的NS2真核表达质粒转染CRFK细胞同时设立空载体转染对照组，每组各三个样本于转染后24 h收样进行转录组测序。结果显示：通过比较两组测序结果，发现转染NS2蛋白的试验组细胞存在218个差异表达基因，其中上调基因200个下调基因18个。通过GO富集分析和KEGG富集分析，发现显著差异基因主要集中病毒胞内入侵和免疫逃逸相关信号通路。本研究揭示NS2蛋白对CRFK细胞的内体转运、TGF-β和RIG-I等多个信号通路在转录水平具有明显的调控作用，系统性揭示FCV NS2蛋白对宿主细胞整体转录调控模式，为后期研究FCV与宿主细胞互作提供了新的研究思路。

关键词: 猫杯状病毒, NS2, 转录组学测序, 差异表达基因

Abstract: This study aims to investigate the interaction between FCV NS2 protein and host cells. In this study, a eukaryotic expression plasmid of FCV NS2 was transfected into CRFK cells, with an empty vector as the control. Three samples from each group were collected 24 hours post-transfection for transcriptomic sequencing. Results were as follows: Comparison of the sequencing results revealed 218 differentially expressed genes (DEGs) in the experimental group transfected with NS2 protein, including 200 upregulated genes and 18 downregulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that the significantly differentially expressed genes were mainly involved in intracellular viral invasion and immune evasion pathways. This study reveals that the NS2 protein significantly regulates multiple signaling pathways at the transcriptional level, including endosomal transport, TGF-β, and RIG-I, in CRFK cells, systematically reveals the global transcriptional regulation patterns of host cells by feline calicivirus (FCV) NS2 protein. These findings provide a new perspective for future research on the interactions between FCV and host cells.

Key words: feline calicivirus, NS2, transcriptome sequencing, differentially expressed genes

中图分类号:

S852.659.6

陈尚琛, 董宁宁, 谈晓梅, 李娜, 张琦, 罗茂青, 邓应坤, 庞帅赛, 高嘉溪, 刘光清, 孟春春. 表达猫杯状病毒NS2质粒转染猫肾传代细胞的转录组学分析[J]. 畜牧兽医学报, 2025, 56(12): 6339-6350.

CHEN Shangchen, DONG Ningning, TAN Xiaomei, LI Na, ZHANG Qi, LUO Maoqing, DENG Yingkun, PANG Shuaisai, GAO Jiaxi, LIU Guangqing, MENG Chunchun. Differential Transcriptomic Analysis of Feline Kidney Cells Transfected with Feline Calicivirus NS2 Protein[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(12): 6339-6350.

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表达猫杯状病毒NS2质粒转染猫肾传代细胞的转录组学分析

Differential Transcriptomic Analysis of Feline Kidney Cells Transfected with Feline Calicivirus NS2 Protein

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