禽白血病病毒J亚群感染鸡组织中细胞受体chNHE1的表达分析

doi:10.11843/j.issn.0366-6964.2024.08.034

畜牧兽医学报 ›› 2024, Vol. 55 ›› Issue (8): 3631-3639.doi: 10.11843/j.issn.0366-6964.2024.08.034

禽白血病病毒J亚群感染鸡组织中细胞受体chNHE1的表达分析

张莉¹^,²(), 于蒙蒙², 王颖², 王素艳², 许壮壮², 刘鹏², 陈运通², 祁小乐², 李留安¹^,*(), 高玉龙²^,*()

1. 天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室, 天津 300392
2. 中国农业科学院哈尔滨兽医研究所动物疫病防控全国重点实验室禽免疫抑制病创新团队, 哈尔滨 150069

收稿日期:2023-11-10 出版日期:2024-08-23 发布日期:2024-08-28
通讯作者: 李留安,高玉龙 E-mail:15275309627@163.com;anliuli2003@163.com;gaoyulong@caas.cn
作者简介:张莉(1999-)，女，山东菏泽人，硕士生，主要从事禽免疫抑制病研究，E-mail：15275309627@163.com
基金资助:
国家自然科学基金重点项目(32230105);国家肉鸡产业技术体系项目(CARS-41)

Analysis of Cellular Receptor chNHE1 Expression in Chicken Tissues Infected with Avian Leukosis Virus Subgroup J

Li ZHANG¹^,²(), Mengmeng YU², Ying WANG², Suyan WANG², Zhuangzhuang XU², Peng LIU², Yuntong CHEN², Xiaole QI², Liuan LI¹^,*(), Yulong GAO²^,*()

1. Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China
2. Avian Immunosuppressive Disease Division, State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China

Received:2023-11-10 Online:2024-08-23 Published:2024-08-28
Contact: Liuan LI, Yulong GAO E-mail:15275309627@163.com;anliuli2003@163.com;gaoyulong@caas.cn

摘要/Abstract

摘要：

为了旨在研究鸡钠氢交换蛋白1(chicken sodium hydrogen exchanger isoform 1，chNHE1)在不同类型鸡组织内的表达情况，J亚群禽白血病病毒(subgroup J avian leukosis virus，ALV-J)的组织嗜性及ALV-J感染后组织chNHE1的mRNA变化情况。本研究利用qPCR技术，检测了商品肉鸡、商品蛋鸡和SPF鸡脏器内的chNHE1转录量，ALV-J感染鸡后各组织的病毒载量及组织chNHE1的转录量的变化。结果显示，chNHE1在3种类型鸡的心、肝、脾、肺、肾、盲肠扁桃体、胸腺和法氏囊中均能检测到，但转录量高低存在较大差异，其中，免疫器官内的chNHE1转录量相对较高；SPF鸡接种ALV-J后，心、脾、肾和盲肠扁桃体中的病毒载量相对较高，而肝、肺、胸腺和法氏囊中的病毒载量相对较低；ALV-J感染后，脾、肾和盲肠扁桃体中的chNHE1转录量明显上调，但心中的表达转录量与空白对照相比无明显变化。本研究分析了3种类型鸡chNHE1的组织转录特点及ALV-J感染对组织内chNHE1转录的影响，该研究结果为探究受体chNHE1的转录与ALV-J的组织嗜性的关系提供重要的理论依据。

关键词: J亚群禽白血病病毒, 鸡钠氢交换蛋白1, 组织表达, 转录量变化

Abstract:

This study aimed to investigate the expression of chicken sodium hydrogen exchanger isoform 1 (chNHE1) in different types of chicken tissues, the tissue tropism of subgroup J avian leukosis virus (ALV-J), and the changes in mRNA of chNHE1 after ALV-J infection. We used qPCR to detect the transcription of chNHE1 in the organs of commercial broilers, commercial layers, and SPF chickens, as well as the viral load in various tissues and the changes in chNHE1 transcription in tissues after ALV-J infection. The results showed that chNHE1 was transcripted in the heart, liver, spleen, lung, kidney, cecal tonsil, thymus, and bursa in all three types of chickens, but the transcription levels varied greatly, with relatively higher transcription levels in the immune organs. After SPF chickens were infected with ALV-J, the viral load was relatively higher in the heart, spleen, kidney, and cecal tonsils, while it was relatively lower in the liver, lung, thymus, and bursa. The transcription of chNHE1 in the spleen, kidney, and cecal tonsils was significantly upregulated after ALV-J infection, but there was no significant change in the transcription in the heart compared to the control group. This study analyzed the tissue transcription characteristics of chNHE1 in three types of chicken and the effect of ALV-J infection on the transcription chNHE1 expression in tissues, providing an important theoretical basis for exploring the relationship between the expressiontranscription of the receptor chNHE1 and the tissue tropism of ALV-J.

Key words: subgroup J avian leukosis virus, chicken sodium hydrogen exchanger isoform 1, tissue expression, transcriptional changes

中图分类号:

S852.659.3

张莉, 于蒙蒙, 王颖, 王素艳, 许壮壮, 刘鹏, 陈运通, 祁小乐, 李留安, 高玉龙. 禽白血病病毒J亚群感染鸡组织中细胞受体chNHE1的表达分析[J]. 畜牧兽医学报, 2024, 55(8): 3631-3639.

Li ZHANG, Mengmeng YU, Ying WANG, Suyan WANG, Zhuangzhuang XU, Peng LIU, Yuntong CHEN, Xiaole QI, Liuan LI, Yulong GAO. Analysis of Cellular Receptor chNHE1 Expression in Chicken Tissues Infected with Avian Leukosis Virus Subgroup J[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(8): 3631-3639.

图/表 5

表 1

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图 4

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引物名称 Primer	引物序列(5′→3′) Primer sequence	片段长度/bp Fragment length
chNHE1-F	CTGAACAAACAGCACCACAAC	291
chNHE1-R	GAGGAGGAAGAGGAAGAAGATG
GAPDH-F	CCCCAATGTCTCTGTTGTTGAC	295
GAPDH-R	CAGCCTTCACTACCCTCTTGAT
ALV-J-ENV-F	TTGCAGGCATTTCTGACTGG	214
ALV-J-ENV-R	ACACGTTTCCTGGTTGTTGC
OVO-F	CACTGCCACTGGGCTCTGT	71
OVO-R	GCAATGGCAATAAACCTCCAA

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禽白血病病毒J亚群感染鸡组织中细胞受体chNHE1的表达分析

Analysis of Cellular Receptor chNHE1 Expression in Chicken Tissues Infected with Avian Leukosis Virus Subgroup J

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