共轭亚油酸诱导的奶牛低脂乳症中脂肪球膜甘油磷脂的组成差异分析

doi:10.11843/j.issn.0366-6964.2022.03.012

摘要/Abstract

摘要： 旨在分析共轭亚油酸(CLA)诱导奶牛低脂乳症对牛乳脂肪球膜甘油磷脂含量的影响。选取体况相近、泌乳中期的10头荷斯坦奶牛进行自身前后对照设计,试验期12 d,第1天至第6天每头牛饲喂基础日粮+CLA 400 g·d^-1,第7天至第12天恢复为饲喂基础日粮,每天记录产奶量及采食量,分析乳成分及乳脂肪球(MFG)粒径参数,对1、7、12 d的乳样采用质谱分析甘油磷脂含量。结果表明,饲喂CLA处理前后对奶牛的产奶量、采食量及乳蛋白、乳糖含量没有显著影响(P>0.05),但显著降低了奶牛的乳脂率(P<0.05)。脂肪球表面积平均直径(D_[3,2])、10%粒子体积径(Dv (10))和50%粒子体积径(Dv (50))的大小均呈现先降低后升高的变化趋势(P<0.05),而比表面积(SSA)则是先升高后降低(P<0.05)。随着饲喂时间的延长,小粒径MFG比例呈现先增加后降低,而大粒径MFG比例则先减小后逐渐增大(P<0.05)。甘油磷脂分析发现,磷脂酰胆碱(PC 28:0、PC 30:0、PC 32:0、PC 34:1、PC 36:4和PC 38:5)和磷脂酰乙醇胺(PE 28:0、PE 30:0、PE 31:1e、PE 32:0、PE 32:2e、PE 33:2e、PE 34:0、PE 34:1、PE 34:2、PE 34:2e、PE 34:4e、PE 34:5e、PE 35:1、PE 35:3e、PE 35:5e、PE 36:2e、PE 36:4、PE 36:5e、PE 38:3、PE 38:4)的含量随着CLA处理前后也呈现先降低后升高的变化趋势,磷脂酰肌醇(PI)的含量增多。上述结果表明,CLA处理影响了奶牛乳脂肪球粒径和膜磷脂的含量和组成。

关键词: 乳脂肪球, 共轭亚油酸, 粒径, 甘油磷脂

Abstract: The purpose of this study was to analyze the content of glycerolphospholipids in fat globule membrane in dairy cows with low-fat depression induced by conjugated linoleic acid (CLA). Ten Holstein cows with similar body conditions and at mid-lactation were selected for their own before and after control design. The test was carried out for 12 days, cows were fed with feeding basal diet +400 g CLA every day from day 1 to day 6, and then changed to the basal diet from day 7 to day 12. Cow performance, milk composition and milk fat globules(MFG) size parameters were measured daily. The milk samples were analyzed for glycerophospholipid on the first、7th and 12th day by mass spectrometry. The results showed that CLA treatment had no significant effect on the milk production, feed intake, milk protein and lactose content of dairy cows (P>0.05), but significantly reduced the milk fat rate of dairy cows (P<0.05). The size of fat globules average surface area diameter (D_[3,2])、10% particle volume diameter (Dv(10)) and 50% particle volume diameter (Dv(50)) all showed a trend of first decreased and then increased (P<0.05), while the specific surface area (SSA) first increased and then decreased (P<0.05). With the extension of feeding time, the small MFG distribution gradually increased and then decreased, while the large MFG first decreased and then gradually increased (P<0.05). Analysis of glycerophospholipids found that the concentration of phosphatidylcholine (PC 28:0, PC 30:0, PC 32:0, PC 34:1, PC 36:4 and PC 38:5) and phosphatidylethanolamine (PE 28:0, PE 30:0, PE 31:1e, PE 32:0, PE 32:2e, PE 33:2e, PE 34:0, PE 34:1, PE 34:2, PE 34:2e, PE 34:4e, PE 34:5e, PE 35:1, PE 35:3e, PE 35:5e, PE 36:2e, PE 36:4, PE 36:5e, PE 38:3, PE 38:4) showed a trend of first decreasing and then increasing. And content of phosphatidylinositol (PI) increased. These results indicate that CLA affect the size of fat globules and content and composition of phospholipids in milk fat globule membrane of dairy cows.

Key words: milk fat globules, conjugated linoleic acid, particle size, glycerophospholipids

中图分类号:

S823.91

杨静娜, 胡明月, 卢文燕, 黄啟雪, 未安琪, 王月影, 钟凯, 韩立强. 共轭亚油酸诱导的奶牛低脂乳症中脂肪球膜甘油磷脂的组成差异分析[J]. 畜牧兽医学报, 2022, 53(3): 791-801.

YANG Jingna, HU Mingyue, LU Wenyan, HUANG Qixue, WEI Anqi, WANG Yueying, ZHONG Kai, HAN Liqiang. Analysis of Milk Fat Globule Membrane Glycerolphospholipids with Low-fat Depression Induced by Conjugated Linoleic Acid in Dairy Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 791-801.

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