栽培一枝蒿粗多糖佐剂增强口蹄疫疫苗的皮下免疫效果

doi:10.11843/j.issn.0366-6964.2022.01.031

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (1): 315-323.doi: 10.11843/j.issn.0366-6964.2022.01.031

栽培一枝蒿粗多糖佐剂增强口蹄疫疫苗的皮下免疫效果

翁翔¹, 张爱莲^1*, 李泉晓¹, 吴道澄², 曹辉³

1. 新疆大学生命科学与技术学院, 新疆生物资源与基因工程重点实验室, 乌鲁木齐 830046;
2. 西安交通大学生命科学与技术学院, 西安 710000;
3. 天康生物股份有限公司, 乌鲁木齐 830000

收稿日期:2021-04-23 出版日期:2022-01-23 发布日期:2022-01-26
通讯作者: 张爱莲,主要从事新型疫苗佐剂研究,E-mail:xjzal@163.com
作者简介:翁翔(1995-),男,新疆伊犁人,硕士生,主要从事新型疫苗佐剂研究,E-mail:alphaweng@163.com
基金资助:
新疆维吾尔自治区科技援疆项目（2020E0232）；国家自然科学基金（31960164）

Immuno-enhancement Effects of Cultivated Artemisia rupestris L. Crude Polysaccharide on Foot-and-mouth Disease Vaccine via Subcutaneous Route

WENG Xiang¹, ZHANG Ailian^1*, LI Quanxiao¹, WU Daocheng², CAO Hui³

1. College of Life Science and Technology, Xinjiang University, Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, Urumqi 830046, China;
2. College of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710000, China;
3. Tecon Biology Co., Ltd., Urumqi 830000, China

Received:2021-04-23 Online:2022-01-23 Published:2022-01-26

摘要/Abstract

摘要： 旨在评价新疆栽培一枝蒿粗多糖（cultivated Artemisia rupestris L.crude polysaccharides，CARCP）对口蹄疫灭活疫苗（foot and mouth disease vaccine，FMDV）的免疫佐剂活性，确定多糖佐剂的有效性。选用不同剂量的CARCP与FMDV配伍，通过皮下途径免疫ICR小鼠。采用间接ELISA检测FMDV特异性抗体水平，流式细胞仪检测免疫后小鼠脾和淋巴结中T细胞亚群，以及脾中树突状细胞表面分子和调节性T细胞的表达情况。每周称量小鼠体重，观察免疫后小鼠的生长状况。结果表明，与FMDV单独免疫对照组相比，中剂量和高剂量的CARCP可以诱导产生更高水平的FMDV特异性IgG、IgG1和IgG2a （P<0.01）。中剂量的CARCP显著增加小鼠脾和淋巴结中CD4、CD8、CD44 T细胞百分比（P<0.05）。同时，中剂量的CARCP可以显著促进脾树突状细胞CD86和MHC-II分子的表达（P<0.05），并显著下调CD4⁺CD25⁺Foxp3⁺T细胞水平（P<0.05）。CARCP对免疫后小鼠生长和体重无显著影响（P>0.05）。综上表明，CARCP作为FMDV佐剂可以促进DC成熟，抑制调节性T细胞（regulatory T cells，Tregs）表达，增强FMDV特异性的体液和细胞免疫应答，具有良好的佐剂效果，为口蹄疫灭活疫苗多糖佐剂的开发提供一定的研究基础。

关键词: 新疆栽培一枝蒿, 粗多糖, 佐剂, 口蹄疫灭活疫苗

Abstract: The purpose of this study was to evaluate the adjuvant effects of cultivated Artemisia rupestris L. crude polysaccharides (CARCP) on inactivated foot-and-mouth disease vaccine (FMDV). ICR mice were subcutaneously administrated with different doses of CARCP combined with FMDV. ELISA was used for the detection of FMDV-specific antibodies. T cell subsets in spleen and lymph nodes, and the expression of dendritic cell surface molecules as well as regulatory T cells (Tregs) in spleen were detected by flow cytometry. Mice were weighed every week, and observed the growth condition of mice after immunization. Compared with FMDV alone, higher levels of FMDV-specific IgG, IgG1 and IgG2a were induced by both medium-dose and high-dose of CARCP (P<0.01). Medium-dose of CARCP not only significantly increased the percentage of CD4, CD8, and CD44 T cells in the spleen and lymph nodes of mice (P<0.05), but also promoted the expression of CD86 and MHC-II in DC (P<0.05), whereas the CD4⁺CD25⁺Foxp3⁺ T cells were significantly down-regulated (P<0.05). There was no noticeable influence on the growth and body weight of immunized mice (P>0.05). The results suggested that CARCP as an adjuvant for FMDV could augment the specific humoral and cellular immune responses via inducing DC maturation, inhibiting Tregs-level, which provided a basis for the development of polysaccharide adjuvant of FMDV.

Key words: Artemisia rupestris L., crude polysaccharide, adjuvant, foot-and-mouth disease vaccine

中图分类号:

S852.42

翁翔, 张爱莲, 李泉晓, 吴道澄, 曹辉. 栽培一枝蒿粗多糖佐剂增强口蹄疫疫苗的皮下免疫效果[J]. 畜牧兽医学报, 2022, 53(1): 315-323.

WENG Xiang, ZHANG Ailian, LI Quanxiao, WU Daocheng, CAO Hui. Immuno-enhancement Effects of Cultivated Artemisia rupestris L. Crude Polysaccharide on Foot-and-mouth Disease Vaccine via Subcutaneous Route[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 315-323.

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栽培一枝蒿粗多糖佐剂增强口蹄疫疫苗的皮下免疫效果

Immuno-enhancement Effects of Cultivated Artemisia rupestris L. Crude Polysaccharide on Foot-and-mouth Disease Vaccine via Subcutaneous Route

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