高效赤红球菌协同CpG ODN增强鸡禽流感疫苗的免疫效力

doi:10.11843/j.issn.0366-6964.2022.12.022

畜牧兽医学报 ›› 2022, Vol. 53 ›› Issue (12): 4367-4378.doi: 10.11843/j.issn.0366-6964.2022.12.022

高效赤红球菌协同CpG ODN增强鸡禽流感疫苗的免疫效力

潘伟雄¹, 陈钰怡¹, 赵锃珏¹, 冯赛祥², 仇微红³, 叶贺佳³, 张玲华^1*

1. 华南农业大学生命科学学院, 广东省农业生物蛋白质功能与调控重点实验室, 广州 510642;
2. 华南农业大学兽医学院, 广州 510642;
3. 广州市华南农大生物药品有限公司, 广州 511300

收稿日期:2022-05-30 出版日期:2022-12-23 发布日期:2022-12-25
通讯作者: 张玲华，主要从事微生物与免疫研究，E-mail：lhzhang@scau.edu.cn
作者简介:潘伟雄(2001-)，男，广东佛山人，本科生，主要从事微生物与免疫研究，E-mail：biebie@stu.scau.edu.cn；陈钰怡(2002-)，女，广东东莞人，本科生，主要从事微生物与免疫研究，E-mail：chenyuyi122004@foxmail.com。潘伟雄和陈钰怡为同等贡献作者
基金资助:
广州市科技计划——民生科技项目(202002020056)

Rhodococcus ruber Synergizes Effectively with CpG ODN to Enhance the Immune Efficacy of the Bird Flu Vaccine in Chickens

PAN Weixiong¹, CHEN Yuyi¹, ZHAO Zengjue¹, FENG Saixiang², QIU Weihong³, YE Hejia³, ZHANG Linghua^1*

1. Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University, Guangzhou 510642, China;
2. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
3. Guangzhou South China Biological Medicine, Co., Ltd., Guangzhou 511300, China

Received:2022-05-30 Online:2022-12-23 Published:2022-12-25

摘要/Abstract

摘要： 禽类疫苗存在免疫效率低、免疫持续时间短和细胞免疫刺激不足等问题，新型禽类疫苗佐剂亟待开发。作者的前期研究证实，CpG ODN具有高度的安全性以及良好的细胞和体液免疫刺激功能，然而，CpG ODN的生产和使用成本十分高昂，尚未用于禽类疫苗佐剂。为了降低成本并提升功效，基于TLR2和TLR21潜在的协同关系，本研究以禽流感疫苗为模式疫苗，探究了赤红球菌和CpG ODN协同刺激免疫的关系。通过HI试验、细胞增殖试验和相关细胞因子表达量的检测，证实了该复合佐剂具有更强大的体液免疫和细胞免疫提升功效，能提升Th 1型免疫反应，免疫保护持续时间长，同时还能增强系统和局部免疫。通过检测受体的表达量，确证了CpG ODN与赤红球菌之间存在着协同关系。此外，在CpG ODN剂量减半的复合佐剂刺激试验中，发现由于协同关系的存在，CpG ODN半剂量的复合佐剂仍具有良好的免疫刺激效果。本研究为进一步降低CpG制剂的生产和使用成本做了先行探究，为CpG制剂高效且成本可控地应用于禽类动物养殖，乃至畜牧养殖奠定了基础。

关键词: 高效佐剂, CpG ODN, 赤红球菌, Th1型免疫

Abstract: Avian vaccines have problems of low immunization efficiency, short immunization duration and insufficient cellular immune stimulation. New avian vaccine adjuvants need to be developed and used to solve these problems. As reported in our former studies, CpG ODN has high safety and good cellular and humoral immune stimulation functions. However, the expensive cost of CpG ODN prevents its promotion in avian vaccines. In order to reduce cost and improve efficacy, based on the potential synergistic relationship between TLR2 and TLR21, this study investigated the relationship between Rhodococcus ruber and CpG ODN to synergistically stimulate immunity by utilizing the avian influenza vaccine as a model vaccine. Through the HI test, cell proliferation test and detection of the expression of related cytokines, we confirmed that the compound adjuvant which induced Th1 immunity has stronger humoral immunity and cellular immunity, a long-last time protection, as well as systemic and local immunity enhancement. By examining the relative expression levels of the receptors, we confirmed that there is a synergistic relationship between CpG ODN and Rhodococcus ruber. In addition, in the compound adjuvant stimulation experiment of halving the dose of CpG ODN, we found that due to the existence of synergy, the compound adjuvant with half dose CpG ODN still had good immune stimulation effect. This study made a preliminary exploration for further reducing the production and use costs of CpG preparations, and laid a foundation for the efficient and cost-controllable promotion of CpG preparations in poultry animal breeding and even animal husbandry.

Key words: effective adjuvant, CpG ODN, Rhodococcus ruber, Th1 immunity

中图分类号:

潘伟雄, 陈钰怡, 赵锃珏, 冯赛祥, 仇微红, 叶贺佳, 张玲华. 高效赤红球菌协同CpG ODN增强鸡禽流感疫苗的免疫效力[J]. 畜牧兽医学报, 2022, 53(12): 4367-4378.

PAN Weixiong, CHEN Yuyi, ZHAO Zengjue, FENG Saixiang, QIU Weihong, YE Hejia, ZHANG Linghua. Rhodococcus ruber Synergizes Effectively with CpG ODN to Enhance the Immune Efficacy of the Bird Flu Vaccine in Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4367-4378.

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高效赤红球菌协同CpG ODN增强鸡禽流感疫苗的免疫效力

Rhodococcus ruber Synergizes Effectively with CpG ODN to Enhance the Immune Efficacy of the Bird Flu Vaccine in Chickens

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