牦牛源正呼肠孤病毒2型的检测和分离鉴定

doi:10.11843/j.issn.0366-6964.2021.08.030

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (8): 2361-2368.doi: 10.11843/j.issn.0366-6964.2021.08.030

• 研究简报 • 上一篇

牦牛源正呼肠孤病毒2型的检测和分离鉴定

吕珽¹, 陈虹吟¹, 汤承^1,2, 岳华^1,2*

1. 西南民族大学畜牧兽医学院, 成都 610041;
2. 动物医学四川省高校重点实验室, 成都 610041

收稿日期:2020-12-15 出版日期:2021-08-23 发布日期:2021-08-21
通讯作者: 岳华,主要从事动物病原微生物研究,E-mail:yhua900@163.com
作者简介:吕珽(1995-),女,四川内江人,硕士,主要从事动物病原微生物研究,E-mail:965357868a@sina.com;陈虹吟(1995-),女,云南曲靖人,硕士,主要从事动物病原微生物研究,E-mail:437013414@qq.com。
基金资助:
西南民族大学新发动物疫病创新团队（2020 NTD02）；动物医学四川省高等学校重点实验室平台项目（2020PTJS29001）

Detection, Isolation and Identification of Mammalian Orthoreovirus Serotype 2 in Yaks

Lü Ting¹, CHEN Hongyin¹, TANG Cheng^1,2, YUE Hua^1,2*

1. College of Animal Science and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China;
2. Key Laboratory of Veterinary Medicine of Universities of Sichuan Province, Chengdu 610041, China

Received:2020-12-15 Online:2021-08-23 Published:2021-08-21

摘要/Abstract

摘要： 旨在调查川西北牦牛哺乳动物正呼肠孤病毒（MRV）的感染情况并分离病毒。采用RT-PCR方法，对采自川西北15个牧场的72份牦牛腹泻粪便样本和其中5个牧场的15份腹泻牦牛血清样本进行MRV检测，阳性样本进一步用分型PCR确定其血清型。结果显示，粪便样本中MRV检出率为20.83%（15/72），血清2型的比例为60%（9/15）；血清样本中MRV检出率为40%（6/15），血清2型的比例为83.33%（5/6）；未检测到其他血清型。成功地从腹泻粪便中分离到1株MRV血清2型毒株（TCID₅₀为4×10^-8.56·mL^-1），并获得长度为23 587 bp的分离株全基因组，该分离株与中国猪源毒株的遗传关系最近；与GenBank中所有的MRV S1基因相比，该分离株有4个独特的氨基酸突变。本研究从牦牛中检测到MRV，并分离到1株牛源MRV血清2型毒株，为进一步研究MRV血清2型生物学特性奠定了基础。

关键词: 牦牛, 哺乳动物正呼肠孤病毒, 检测, 血清2型, 分离鉴定

Abstract: This study aimed to investigate the infection of mammalian orthoreovirus (MRV) in Northwest Sichuan yaks and isolate MRV from positive samples. 72 fecal samples of diarrhea yaks from 15 pastures in northwest Sichuan and 15 serum samples of diarrhea yaks from 5 pastures were collected for detecting MRV by PT-PCR, and the positive samples were further serotyped by PCR assay. The results showed that the detection rates of MRV in diarrhea samples and serum samples were 20.83% (15/72) and 40% (6/15), respectively; and MRV2 was 60% (9/15) in MRV-positive diarrhea samples and 83.33% (5/6) in MRV-positive serum samples, respectively; other serotypes were not detected. One strain of MRV2 (TCID₅₀ is 4×10^-8.56·mL^-1) was isolated from a fecal sample, and its complete genome was successfully sequenced with 23 587 bp in length. In phylogenetic analysis of MRV genomes, the strain yak/AB14/2018 is closely related to China swine strains. Compared with all S1 sequences availble in GenBank, this strain shared 4 amino acids variations. In conclusion, MRV was detected from yaks, and one MRV2 strain was successfully isolated, contributing to further research the biological characteristics of MRV2.

Key words: yak, mammalian orthoreovirus, detection, serotype 2, isolation

中图分类号:

S852.659.4

吕珽, 陈虹吟, 汤承, 岳华. 牦牛源正呼肠孤病毒2型的检测和分离鉴定[J]. 畜牧兽医学报, 2021, 52(8): 2361-2368.

LÜ Ting, CHEN Hongyin, TANG Cheng, YUE Hua. Detection, Isolation and Identification of Mammalian Orthoreovirus Serotype 2 in Yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2361-2368.

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牦牛源正呼肠孤病毒2型的检测和分离鉴定

Detection, Isolation and Identification of Mammalian Orthoreovirus Serotype 2 in Yaks

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