GADD45β蛋白抑制ALV-J在DF-1细胞中的复制

doi:10.11843/j.issn.0366-6964.2021.06.020

畜牧兽医学报 ›› 2021, Vol. 52 ›› Issue (6): 1662-1669.doi: 10.11843/j.issn.0366-6964.2021.06.020

GADD45β蛋白抑制ALV-J在DF-1细胞中的复制

陈胜¹, 廖志宏¹, 谢姿¹, 陈峰¹, 谢青梅¹, 舒薇^2*

1. 华南农业大学动物科学学院畜禽健康养殖与疾病防控实验室, 广州 510642;
2. 华南农业大学测试中心, 广州 510642

收稿日期:2020-07-06 出版日期:2021-06-23 发布日期:2021-06-22
通讯作者: 舒薇,主要从事蛋白质与酶的结构功能、生物显微成像与成分分析检测技术研究,E-mail:shuwei@scau.edu.cn
作者简介:陈胜(1996-),男,安徽安庆人,硕士生,主要从事畜牧兽医相关研究,E-mail:1275094142@qq.com

GADD45β Suppresses the Replication of Avian Leukosis Virus Subgroup J in DF-1 Cells

CHEN Sheng¹, LIAO Zhihong¹, XIE Zi¹, CHEN Feng¹, XIE Qingmei¹, SHU Wei^2*

1. Laboratory of Livestock Healthy Cultivation & Disease Prevention and Control Research, College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. Instrumental Analysis & Research Center of South China Agricultural University, Guangzhou 510642, China

Received:2020-07-06 Online:2021-06-23 Published:2021-06-22

摘要/Abstract

摘要： 生长阻滞和DNA损伤诱生蛋白45β（growth arrest and DNA damage 45β，GADD45β）参与多种细胞信号通路，在病毒感染过程中发挥重要作用，但在禽白血病病毒（avian leukosis virus，ALV）感染中研究较少。本研究中，用J亚群禽白血病病毒（ALV-J）感染DF-1细胞，通过荧光定量PCR和Western blot检测GADD45β表达水平。此外，在过表达GADD45β和干扰GADD45β的情况下，通过Western blot、间接免疫荧光试验和ELISA检测GADD45β对ALV-J复制的影响。结果表明，ALV-J感染DF-1细胞能显著上调GADD45β表达水平（P<0.05）。过表达GADD45β后，ALV-J的蛋白表达水平显著降低（P<0.05），荧光信号强度也明显低于对照组。然而，干扰GADD45β后，ALV-J的复制水平显著上调（P<0.05），说明GADD45β可以抑制ALV-J病毒复制。本研究首次发现GADD45β具有抑制ALV-J病毒复制的功能，为抗ALV-J的研究提供了新的思路和理论基础。

关键词: 生长阻滞和DNA损伤诱生蛋白45β, GADD45β, ALV-J, 病毒复制

Abstract: Growth arrest and DNA damage 45β (GADD45β) is involved in various cellular signaling pathways and plays an important role in viral infection, but there are few studies on avian leukosis virus (ALV) infection. In this study, we used RT-qPCR and Western blot to detect the expression level of GADD45β in DF-1 cells infected with ALV-J. To investigate the effect of GADD45β on the replication of ALV-J, immunofluorescence, Western blot and ELISA were used to detect the effect of overexpression of GADD45β and interference of GADD45β on ALV-J replication. The results showed that the protein level of ALV-J was decreased after overexpression of GADD45β. However, when interfering the expression of GADD45β, the replication of ALV-J showed the opposite effect, indicating that GADD45β inhibited the replication of ALV-J. This study found for the first time that GADD45β has the effect of inhibiting the replication of ALV-J, which is of great significance and value for ALV-J-related research.

Key words: growth arrest and DNA damage 45β, GADD45β, ALV-J, virus replication

中图分类号:

S852.659.3

陈胜, 廖志宏, 谢姿, 陈峰, 谢青梅, 舒薇. GADD45β蛋白抑制ALV-J在DF-1细胞中的复制[J]. 畜牧兽医学报, 2021, 52(6): 1662-1669.

CHEN Sheng, LIAO Zhihong, XIE Zi, CHEN Feng, XIE Qingmei, SHU Wei. GADD45β Suppresses the Replication of Avian Leukosis Virus Subgroup J in DF-1 Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1662-1669.

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GADD45β蛋白抑制ALV-J在DF-1细胞中的复制

GADD45β Suppresses the Replication of Avian Leukosis Virus Subgroup J in DF-1 Cells

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