急性睡眠剥夺对小鼠血浆褪黑激素水平和下丘脑钟基因表达的影响

doi:10.11843/j.issn.0366-6964.2020.10.030

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (10): 2609-2612.doi: 10.11843/j.issn.0366-6964.2020.10.030

• 研究简报 • 上一篇

急性睡眠剥夺对小鼠血浆褪黑激素水平和下丘脑钟基因表达的影响

杨悦, 郭泽明, 韩昊书, 李伟天, 高婷, 王心彤, 陈耀星*

中国农业大学动物医学院, 北京 100193

收稿日期:2020-04-05 出版日期:2020-10-25 发布日期:2020-10-26
通讯作者: 陈耀星,主要从事神经生物学研究,E-mail:yxchen@cau.edu.cn
作者简介:杨悦(1997-),男,湖北武汉人,本科生,主要从事动物医学研究,E-mail:2621313293@qq.com
基金资助:
国家级大学生创新计划（201810019098）；北京市自然科学基金（6182018）

Effect of Acute Sleep Deprivation on Melatonin Level of Plasma and Clock Gene Expression of Hypothalamus in Mice

YANG Yue, GUO Zeming, HAN Haoshu, LI Weitian, GAO Ting, WANG Xintong, CHEN Yaoxing*

Laboratory of Anatomy of Domestic Animals, College of Animal Medicine, China Agricultural University, Beijing 100193, China

Received:2020-04-05 Online:2020-10-25 Published:2020-10-26

摘要/Abstract

摘要： 旨在探究睡眠剥夺后小鼠血浆褪黑激素（MT）水平和下丘脑钟基因表达的变化。将72只小鼠随机分为对照组与睡眠剥夺试验组（n=36），试验组小鼠接受水平台法连续72 h睡眠剥夺。在试验结束后当日每隔4 h（CT4、8、12、16、20和24时）采血测定血浆MT含量，并取下丘脑以RT-PCR方法测定钟基因mClock、mBmal1、mCry1/2、mPer1～3、mRorβ和mReverbα表达量。结果显示，相较于对照组，试验组小鼠血浆MT水平昼夜节律紊乱。在下丘脑钟基因的表达量上，mClock、mRorβ和mReverbα的中值显著下降（P<0.05），而mPer1和mPer2中值显著增加（P<0.01）；对于昼夜节律，mCry1和mCry2的昼夜节律消失；对于钟基因的相位，mBmal1和mClock相位提前，而mRorβ和mPer1~3相位延后。结果提示，小鼠睡眠剥夺3 d可致MT分泌和下丘脑钟基因表达节律紊乱。

关键词: 睡眠剥夺, 褪黑激素, 钟基因, 下丘脑, 昼夜节律

Abstract: This study was conducted to investigate the changes in plasma Melatonin (MT) level and hypothalamic clock gene expression in mice after sleep deprivation (SD). Seventy-two mice were randomly divided into SD group and non-sleep-deprived control group (n=36). The SD mice were subjected to a modified multiple platform water path for 72 h sleep deprivation. After that, blood samples were taken every four hours (CT4,8,12,16,20 and 24) for the detection of plasma MT content, and hypothalamus samples were collected for the detection of the expressions of clock genes including mClock, mBmal1, mCry1/2, mPer1-3, mRorβ and mReverbα. The results showed that compared with control group, circadian rhythm of plasma MT was disordered in SD group. The median values of mClock, mRorβ and mReverbα in the hypothalamus decreased significantly (P<0.05), but mPer1 and mPer2 increased significantly (P<0.01); the circadian rhythms of mCry1 and mCry2 disappeared; the expression rhythms of mBmal1, and mClock were advanced in phase, while mRorβ and mPer1-3’s were delayed. These results suggested that acute sleep deprivation of 3 d could cause disorders of MT secretion and hypothalamic clock gene expression in mice.

Key words: sleep deprivation, melatonin, clock gene, hypothalamus, circadian rhythm

中图分类号:

S856.5

杨悦, 郭泽明, 韩昊书, 李伟天, 高婷, 王心彤, 陈耀星. 急性睡眠剥夺对小鼠血浆褪黑激素水平和下丘脑钟基因表达的影响[J]. 畜牧兽医学报, 2020, 51(10): 2609-2612.

YANG Yue, GUO Zeming, HAN Haoshu, LI Weitian, GAO Ting, WANG Xintong, CHEN Yaoxing. Effect of Acute Sleep Deprivation on Melatonin Level of Plasma and Clock Gene Expression of Hypothalamus in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2609-2612.

参考文献 13

[1]	KUN X,HONG H C,SUBRAMANIAN P.Melatonin and sleep[J].Biol Rhythm Res,2019,50(3):490-493.
[2]	TAKAESU Y,FUTENMA K,KOBAYASHI M,et al.A preliminary study on the relationships between diurnal melatonin secretion profile and sleep variables in patients emergently admitted to the coronary care unit[J].Chronobiol Int,2015,32(6):875-879.
[3]	HASTINGS M H,MAYWOOD E S,BRANCACCIO M.Generation of circadian rhythms in the suprachiasmatic nucleus[J].Nat Rev Neurosci,2018,19(8):453-469.
[4]	MATTAM U,JAGOTA A.Differential role of melatonin in restoration of age-induced alterations in daily rhythms of expression of various clock genes in suprachiasmatic nucleus of male Wistar rats[J].Biogerontology,2014,15(3):257-268.
[5]	DKHISSI-BENYAHYA O,COUTANSON C,KNOBLAUCH K,et al.The absence of melanopsin alters retinal clock function and dopamine regulation by light[J].Cell Mol Life Sci,2013,70(18):3435-3447.
[6]	SINGH D,RANI S,KUMAR V.Daily expression of six clock genes in central and peripheral tissues of a night-migratory songbird:evidence for tissue-specific circadian timing[J].Chronobiol Int,2013,30(10):1208-1217.
[7]	GAO T,WANG Z X,DONG Y L,et al.Role of melatonin in sleep deprivation-induced intestinal barrier dysfunction in mice[J].J Pineal Res,2019,67(1):e12574.
[8]	SAHA S,SINGH K M,GUPTA B B P.Melatonin synthesis and clock gene regulation in the pineal organ of teleost fish compared to mammals:Similarities and differences[J].Gen Comp Endocrinol,2019,279:27-34.
[9]	YAMANAKA Y,SUZUKI Y,TODO T,et al.Loss of circadian rhythm and light-induced suppression of pineal melatonin levels in Cry1 and Cry2 double-deficient mice[J].Genes Cells,2010,15(10):1063-1071.
[10]	WISOR J P,PASUMARTHI R K,GERASHCHENKO D,et al.Sleep deprivation effects on circadian clock gene expression in the cerebral cortex parallel electroencephalographic differences among mouse strains[J].J Neurosci,2008,28(28):7193-7201.
[11]	DEBOER T,DÉTÁRI L,MEIJER J H.Long term effects of sleep deprivation on the mammalian circadian pacemaker[J].Sleep,2007,30(3):257-262.
[12]	YAMANAKA Y,YAMADA Y,HONMA K I,et al.Cryptochrome deficiency enhances transcription but reduces protein levels of pineal Aanat[J].J Mol Endocrinol,2018,61(4):219-229.
[13]	BINKLEY S.Pineal gland biorhythms:N-acetyl-transferase in chickens and rats[J].Fed Proc,1976,35(12):2347-2352.

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急性睡眠剥夺对小鼠血浆褪黑激素水平和下丘脑钟基因表达的影响

Effect of Acute Sleep Deprivation on Melatonin Level of Plasma and Clock Gene Expression of Hypothalamus in Mice

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