猪囊等孢球虫孢子化与未孢子化卵囊的差异表达蛋白质组学分析

doi:10.11843/j.issn.0366-6964.2020.09.019

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (9): 2216-2226.doi: 10.11843/j.issn.0366-6964.2020.09.019

猪囊等孢球虫孢子化与未孢子化卵囊的差异表达蛋白质组学分析

杨守深¹, 孙晓双^1,2, 邱云飞^1,3, 杨飞^1,4, 唐杰¹, 连文健¹, 黄翠琴^1*, 邱泓铨^1*

1. 龙岩学院生命科学学院/福建省家畜传染病防治与生物技术重点实验室, 龙岩 364012;
2. 福建农林大学动物科学学院, 福州 350002;
3. 中国农业科学院兰州兽医研究所, 兰州 730046;
4. 西藏农牧学院动物科学学院, 林芝 860000

收稿日期:2020-02-24 出版日期:2020-09-25 发布日期:2020-09-25
通讯作者: 黄翠琴,主要从事病原分子生物学研究,E-mail:cuiqinh@126.com;邱泓铨,主要从事病原分子生物学研究,E-mail:3321766553@qq.com
作者简介:杨守深(1982-),男,福建南平人,副教授,博士,主要从事病原分子生物学研究,E-mail:31526230@qq.com;Tel:0597-2797255
基金资助:
福建省科技厅自然科学基金面上项目（2016J01711）；全国大学生创新创业训练计划项目（201811312001）；龙岩市连城县奇迈科技创新基金项目（2018LCQMJ01）；中央引导地方科技发展专项（2019L3011）；福建省重大专项（2019NZ09005）；龙岩市奇迈科技创新基金项目（2017QM0203）

Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis

YANG Shoushen¹, SUN Xiaoshuang^1,2, QIU Yunfei^1,3, YANG Fei^1,4, TANG Jie¹, LIAN Wenjian¹, HUANG Cuiqin^1*, CHIU Hungchuan^1*

1. Longyan University&Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan 364012, China;
2. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
3. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China;
4. College of Animal Science, Tibetan Agricultural and Animal Husbandry College, Linzhi 860000, China

Received:2020-02-24 Online:2020-09-25 Published:2020-09-25

摘要/Abstract

摘要： 通过比较猪囊等孢球虫孢子化卵囊与未孢子化卵囊的差异表达蛋白质，以期从蛋白质组学角度研究其发育机制，并为此类球虫的发育机制及该病的免疫预防或化学防治找到新的“关键”分子奠定基础。首先建立哺乳仔猪的猪囊等孢球虫感染模型，然后运用iTRAQ技术，结合LC-MS/MS分析差异表达蛋白质，并采用荧光定量PCR验证iTRAQ数据。结果共鉴定出174个蛋白，差异表达蛋白40个，其中38个蛋白上调表达，2个蛋白下调表达，差异蛋白主要涉及代谢、抗生素的生物合成、次生代谢产物的生物合成和糖酵解/糖异生过程等通路。研究结果为阐明猪囊等孢球虫孢子化发育机制以及发现新的生物标志物提供参考。

关键词: 猪囊等孢球虫, 孢子化卵囊, 未孢子化卵囊, iTRAQ

Abstract: The differentially expressed proteins between sporulated oocyst and unsporulated oocyst of Cystoisospora suis strains were screened and compared. In this study, the C. suis infection model of piglets were established and a quantitative proteomic analysis between sporulated oocyst and unsporulated oocyst of C. suis were conducted by iTRAQ labeling and LC-MS/MS. Part of the iTRAQ results were validated by qRT-PCR. Total 174 proteins were identified, among which 40 proteins were differentially expressed, 38 proteins were upregulated and 2 proteins were downregulated. The differentially expressed proteins were mainly involved in metabolism, antibiotics biosynthesis, secondary metabolites biosynthesis and glycolysis/gluconeogenesis pathways. The results of this study provide reference for elucidating the developmental mechanism of C. suis sporulated oocyst and facilitated discovering a new biomarker for C. suis.

Key words: Cystoisospora suis, sporulated oocyst, unsporulated oocyst, iTRAQ

中图分类号:

S852.723

杨守深, 孙晓双, 邱云飞, 杨飞, 唐杰, 连文健, 黄翠琴, 邱泓铨. 猪囊等孢球虫孢子化与未孢子化卵囊的差异表达蛋白质组学分析[J]. 畜牧兽医学报, 2020, 51(9): 2216-2226.

YANG Shoushen, SUN Xiaoshuang, QIU Yunfei, YANG Fei, TANG Jie, LIAN Wenjian, HUANG Cuiqin, CHIU Hungchuan. Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(9): 2216-2226.

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猪囊等孢球虫孢子化与未孢子化卵囊的差异表达蛋白质组学分析

Differential Proteomic Analysis between Sporulated Oocyst and Unsporulated Oocyst of Cystoisospora suis

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