CRISPR/Cas9技术及其在猪基因编辑中的应用

doi:10.11843/j.issn.0366-6964.2020.03.001

摘要/Abstract

摘要： CRISPR/Cas9（clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9）是一种新型基因编辑系统，是由细菌和古细菌对抗入侵病毒及外源DNA的适应性免疫防御系统演变而来的。该系统具有简易、精准、经济和高效的优点，目前已广泛应用于基因编辑及其相关研究中。本文总结了近几年CRISPR/Cas9技术在猪基因组编辑中的研究进展，并展望了其应用前景。

关键词: CRISPR/Cas9, 基因编辑, 猪, 应用

Abstract: CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9) is a novel editing system, which is originally developed from the bacteria defense immune mechanism against viruses infection and plasmid transfer. As a simple, accurate, economical and efficient tool, this technology has been extensively used in the gene-editing and related research fields. The progress and application of CRISPR/Cas9 technology in pig genome editing in the past few years are summarized in this review, and also discusses its future perspectives in pig breeding and clinical trials.

Key words: CRISPR/Cas9, genome editing, pig, application

中图分类号:

S828.2

刘思远, 卢丹, 唐中林. CRISPR/Cas9技术及其在猪基因编辑中的应用[J]. 畜牧兽医学报, 2020, 51(3): 409-416.

LIU Siyuan, LU Dan, TANG Zhonglin. Research Progress on CRISPR/Cas9 and Its Application in Pigs Genome Editing[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(3): 409-416.

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