甜菜碱对瘦素缺陷小鼠肌肉组织中脂质沉积的影响初探

doi:10.11843/j.issn.0366-6964.2020.01.012

畜牧兽医学报 ›› 2020, Vol. 51 ›› Issue (1): 99-108.doi: 10.11843/j.issn.0366-6964.2020.01.012

甜菜碱对瘦素缺陷小鼠肌肉组织中脂质沉积的影响初探

何劲^1,2, 张锫文^1,2, 堵晶晶^1,2, 肖军森^1,2, 杨东丽³, 王定国⁴, 张顺华^1,2, 朱砺^1,2*

1. 四川农业大学动物科技学院, 成都 611130;
2. 四川农业大学, 畜禽遗传资源发掘与创新利用四川省重点实验室, 成都 611130;
3. 泸州市农业农村局, 泸州 646000;
4. 成都市动物疫病预防控制中心, 成都 610041

收稿日期:2019-06-27 出版日期:2020-01-23 发布日期:2020-01-17
通讯作者: 朱砺,主要从事猪遗传育种研究,E-mail:zhuli7508@163.com
作者简介:何劲(1996-),男,四川安岳人,硕士,主要从事猪遗传育种研究,E-mail:hejin19960812@163.com
基金资助:
“十二五”国家科技支撑计划项目（2015BAD03B01-11）；四川省科技支撑项目（2016NYZ0050）；国家现代农业产业技术体系四川生猪创新团队项目（SCSZTD-3-008）

Preliminary Study on the Effect of Betaine on Lipid Deposition in Muscle Tissue of Mice with Leptin Deficiency

HE Jin^1,2, ZHANG Peiwen^1,2, DU Jingjing^1,2, XIAO Junsen^1,2, YANG Dongli³, WANG Dingguo⁴, ZHANG Shunhua^1,2, ZHU Li^1,2*

1. College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, China;
2. Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, China;
3. Luzhou Bureau of Agricultural and Rural Affairs, Luzhou 646000, China;
4. Chengdu Animal Epidemic Prevention and Control Center, Chengdu 610041, China

Received:2019-06-27 Online:2020-01-23 Published:2020-01-17

摘要/Abstract

摘要： 为探究甜菜碱对瘦素缺陷（ob/ob）小鼠肌肉组织中脂质沉积的影响和潜在机制，本研究将12只同批次6周龄雄性、健康、体重差异不超过1 g的ob/ob小鼠随机均分为两组，分别作为试验组和对照组。其中对照组小鼠饮用纯净水，试验组小鼠饮用的纯净水中含1%甜菜碱。1个月后脱颈处死，采集肌肉样本，利用油红O染色、甘油三酯含量测定检测ob/ob小鼠肌肉组织中脂质沉积情况；利用气象色谱-质谱联用系统分析肌肉组织中脂肪酸组成及含量；利用qRT-PCR方法检测肌肉组织中基因表达的变化。结果显示：与对照组相比，1）补饲甜菜碱后ob/ob小鼠的体重增加量、肌肉组织中脂质沉积、禁饲血糖水平显著降低（P<0.05）；2）补饲甜菜碱后，ob/ob小鼠肌肉中脂肪甘油三酯脂肪酶（adipose triacylglyceride lipase，ATGL）、激素敏感性甘油三酯脂肪酶（hormone-sensitive lipase，HSL）以及葡萄糖转运蛋白4（glucose transpoter 4，GLUT4）基因的表达量极显著升高（P<0.01）；3）补饲甜菜碱后，ob/ob小鼠肌肉中饱和脂肪酸（saturated fatty acids，SFA）和多不饱和脂肪酸（polyunsaturated fatty acids，PUFA）含量显著升高（P<0.05）；4）补饲甜菜碱后，ob/ob小鼠肌肉中脂肪酸合成基因：脂肪酸合成酶（fatty acid synthetase，FAS）表达量显著降低（P<0.05），乙酰辅酶A合成酶（acetyl-CoA synthetase，ACS）表达量无显著性变化（P>0.05）；脂肪酸氧化基因：过氧化物酶体增殖物激活受体α（peroxisome proliferator activated receptor alpha，PPARα）、酰基辅酶A氧化酶（acyl-CoA oxidase2，ACOX2）、长链酰基辅酶A脱氢酶（long chain acyl-CoA dehydrogenase，ACADL）、脂肪酸转位酶（fatty acid transposase， CD36）表达量极显著升高（P<0.01）。结果表明，ob/ob小鼠补饲甜菜碱后，肌肉组织脂肪酸合成基因表达降低、脂肪酸氧化基因表达升高，同时肌肉组织脂肪酸含量发生改变，最终导致肌肉组织脂质减少。

关键词: 甜菜碱, ob/ob小鼠, 肌肉组织, 脂质沉积

Abstract: To investigate the effect of betaine on lipid deposition and its potential mechanism in muscle tissue of mice with leptin deficiency (ob/ob), in this study, twelve 6-week-old healthy ob/ob male mice of the same batch were randomly divided into two groups:experimental group and control group. Their weight difference was less than 1 g. Mice in the control group drank purified water, and mice in the experimental group drank purified water containing 1% betaine. One month later, the mice were killed and muscle samples were collected. Then, lipid deposition in muscles was detected by oil red O staining and triglyceride detection, and fatty acids composition and content in muscles were analyzed by Meteorological Chromatography-Mass Spectrometry System. Also, qRT-PCR was used to measure gene expression levels in muscle. The results showed that,compared with control group:1) The weight gain, lipids deposition and fasting blood glucose were significantly reduced after ob/ob mice were treated with betaine(P<0.05). 2) Betaine supplementation caused the extremely significantly increase in the expression of hormone-sensitive lipase (HSL), adipose triacylglyceride lipase (ATGL) and glucose transpoter 4 (GLUT4) (P<0.01). 3) Betaine supplementation could significantly increase saturated fatty acids (SFA) and polyunsaturated fatty acids (PUFA) content in muscle tissues of ob/ob mice(P<0.05). 4) Betaine supplementation significantly down regulated the expression of fatty acids synthetase (FAS, P<0.05), and up regulated the expression of fatty acid oxidation genes such as peroxisome proliferator activated receptor alpha (PPARα), acyl-CoA oxidase2 (ACOX2), long chain acyl-CoA dehydrogenase (ACADL) and fatty acid transposase (CD36) in muscle of ob/ob mice(P<0.01), while no significant difference was observed in acetyl-CoA synthetase (ACS) expression levels between experimental group and control group(P>0.05). When ob/ob mice were supplemented with betaine for one month, the expression of fatty acid synthesis gene in muscle tissue decreased, the expression of fatty acid oxidation gene increased, and the fatty acid content of muscle tissue changed, which eventually led to the decrease of muscle tissue lipid.

Key words: betaine, ob/ob mice, muscle tissue, lipid deposition

中图分类号:

S816

何劲, 张锫文, 堵晶晶, 肖军森, 杨东丽, 王定国, 张顺华, 朱砺. 甜菜碱对瘦素缺陷小鼠肌肉组织中脂质沉积的影响初探[J]. 畜牧兽医学报, 2020, 51(1): 99-108.

HE Jin, ZHANG Peiwen, DU Jingjing, XIAO Junsen, YANG Dongli, WANG Dingguo, ZHANG Shunhua, ZHU Li. Preliminary Study on the Effect of Betaine on Lipid Deposition in Muscle Tissue of Mice with Leptin Deficiency[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(1): 99-108.

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甜菜碱对瘦素缺陷小鼠肌肉组织中脂质沉积的影响初探

Preliminary Study on the Effect of Betaine on Lipid Deposition in Muscle Tissue of Mice with Leptin Deficiency

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