藏鸡不同发育阶段腿部肌肉组织转录组及microRNA联合分析

doi:10.11843/j.issn.0366-6964.2019.12.004

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (12): 2400-2412.doi: 10.11843/j.issn.0366-6964.2019.12.004

藏鸡不同发育阶段腿部肌肉组织转录组及microRNA联合分析

袁茂^1,2, 江明锋^1,2, 徐亚欧^1,2, 林亚秋^1,2, 蒋小松³, 杨朝武³, 余春林³, 李志雄^1,2*

1. 西南民族大学生命科学与技术学院青藏高原动物遗传资源保护与利用教育部重点实验室, 成都 610041;
2. 西南民族大学生命科学与技术学院青藏高原动物遗传资源保护与利用四川省重点实验室, 成都 610041;
3. 四川省畜牧科学研究院, 成都 610066

收稿日期:2019-05-16 出版日期:2019-12-23 发布日期:2019-12-20
通讯作者: 李志雄,主要从事家禽遗传育种研究,E-mail:lzx4113@126.com
作者简介:袁茂(1994-),男,四川遂宁人,硕士生,主要从事分子遗传学与基因工程研究,E-mail:904641105@qq.com
基金资助:
四川省“十三五”畜禽育种攻关项目（2016NYZ0043）；四川省应用基础项目（2016JY0068）；中央高校基本科研业务费专项资金项目（2018NQN10）

Analysis of Transcriptome and microRNA in Leg Muscle of Tibetan Chicken at Different Developmental Stages

YUAN Mao^1,2, JIANG Mingfeng^1,2, XU Ya'ou^1,2, LIN Yaqiu^1,2, JIANG Xiaosong³, YANG Chaowu³, YU Chunlin³, LI Zhixiong^1,2*

1. Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China;
2. Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization Key Laboratory of Sichuan Province, College of Life Science and Technology, Southwest Minzu University, Chengdu 610041, China;
3. Sichuan Animal Science Academy, Chengdu 610066, China

Received:2019-05-16 Online:2019-12-23 Published:2019-12-20

摘要/Abstract

摘要： 旨在从转录组和miRNA角度探讨藏鸡肌肉发育的调控机制，了解藏鸡肌肉发育的特殊性。本研究对120和150日龄藏鸡的肌肉组织进行转录组和small RNA测序，筛选出两个日龄阶段差异表达的基因和miRNA，并利用qRT-PCR技术对测序结果进行验证。结果，共筛选出1 691个差异表达基因，其中上调基因330个，下调基因1 361个。差异表达miRNA共有22个，其中9个上调，13个下调。随机选择的5个基因和miRNAs的qPCR验证结果表明表达趋势与测序结果一致。GO富集分析显示，在富集前10的条目中，与免疫相关的条目占较大比例。KEGG分析结果显示，在19个显著富集通路中，与免疫相关的通路占较大比例，且83个miRNA的靶基因出现在显著富集通路上。转录组和small RNA测序数据联合分析表明，343个miRNA-mRNA对为负相关调控模式。本研究从多组学层面为进一步理解藏鸡的肌肉发育提供了理论基础。

关键词: 藏鸡, 肌肉发育, 转录组, miRNA

Abstract: The objectives of this study were to investigate the regulation mechanism and particularity of muscle development in Tibetan chicken by RNA-sequencing and miRNA-sequencing. mRNA and small RNA in muscle tissues of Tibetan chicken on 120-and 150-day-old were sequenced. The differentially expressed genes (DEGs) and miRNAs were screened between two stages. The sequencing results were verified by qRT-PCR. A total of 1 691 DEGs were screened, of which 330 genes were up-regulated and 1 361 genes were down-regulated. Twenty two differentially expressed miRNAs were screened, of which 9 miRNAs were up-regulated and 13 miRNAs were down-regulated. qPCR verification results of 5 randomly selected genes and miRNAs showed that the expression trend was consistent with the sequencing results. GO enrichment analysis showed that the terms related to immunization accounted for a large proportion in the top 10 enrichment terms. The KEGG analysis showed that the immune-related pathways accounted for a large proportion in the 19 significant enriched pathways, and 83 miRNA target genes were in the significant enriched pathways. The integrated analysis of transcriptome and small RNA sequencing data indicated that 343 pairs of miRNA-mRNA were in the negatively correlated regulation pattern. This study provides a theoretical basis for further understanding the muscle development of Tibetan chicken from the multi-omics.

Key words: Tibetan chicken, muscle development, transcriptome, miRNA

中图分类号:

S831.2

袁茂, 江明锋, 徐亚欧, 林亚秋, 蒋小松, 杨朝武, 余春林, 李志雄. 藏鸡不同发育阶段腿部肌肉组织转录组及microRNA联合分析[J]. 畜牧兽医学报, 2019, 50(12): 2400-2412.

YUAN Mao, JIANG Mingfeng, XU Ya'ou, LIN Yaqiu, JIANG Xiaosong, YANG Chaowu, YU Chunlin, LI Zhixiong. Analysis of Transcriptome and microRNA in Leg Muscle of Tibetan Chicken at Different Developmental Stages[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(12): 2400-2412.

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藏鸡不同发育阶段腿部肌肉组织转录组及microRNA联合分析

Analysis of Transcriptome and microRNA in Leg Muscle of Tibetan Chicken at Different Developmental Stages

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