Abstract: A single factor duplicate test was designed to investigate whether additional leptin(5 ng/mL·12 h) in adipose cell cultured medium at 12,24,36,48 and 72 hours(12 hour each)has a role on ObRb mRNA expression by competitivePCR. The result showed that the quantity of ObRb mRNA in adipose cell cultured for 12～24 h was significantly higher in the treated groups than that of control group（P<0.01）.Following gradual decreasion ,the expression of ObRb kept a stable level from 48 to 72 h（P>0.05）.It considered that leptin could accelerate the expression of ObRb in the cultured adipose cells with giveen time.

Key words: recombination leptin, adipocyte, Ob-Rb mRNA, in vitro culture