急性热应激对山羊血液生化指标及血淋巴细胞热休克蛋白70家族基因表达的影响

doi:10.11843/j.issn.0366-6964.2019.06.012

Abstract

Abstract: This experiment was conducted to explore the effects of acute heat stress on antioxidant capacity, immune function and expression of HSP70 family genes in blood lymphocytes in goats. Five (12±0.5)-month-old healthy female goats (F₁ of Boer goat×Guanzhong dairy goat) were selected and raised separately in cage in an environment controlled chamber (temperature:20℃, relative humidity:60%), adaptation period lasted for 5 days. At the 6th day, five goats were treated with acute heat stress at 38℃ for 12 h. Blood samples were collected before heat stress (0 h, 20℃) and after 2, 4, 8 and 12 h of heat stress. Serum antioxidant indexes (total antioxidant capacity, superoxide dismutase, glutathione peroxidase activity and malondialdehyde content), serum immune indexes (serum immunoglobulin and cytokine contents) and the expression of HSP70 family member genes (HSPA1A, HSPA6 and HSPA8) in blood lymphocytes were measured by colorimetric method, ELISA and RT-PCR, respectively. The results showed as follows:1) Heat stress time had significant effects on serum antioxidant indexes. Compared with 0 h before heat stress, serum T-AOC (P<0.05), SOD (P<0.05) and GSH-Px (P<0.01) activities decreased significantly after 8 h of heat stress, and MDA content increased significantly after 4 h of heat stress (P<0.05). 2) Heat stress time had also significant influence on serum immune indexes. The contents of serum TNF-α and IL-2 increased significantly after 4 h of heat stress (P<0.05), while the contents of IL-1β and IFN-γ increased significantly after 8 h of heat stress (P<0.05). The contents of IgG (P<0.01), IgM (P<0.01) and IgA (P<0.05) decreased significantly after 4 h of heat stress, while the content of IL-4 decreased significantly after 12 h of heat stress(P<0.01). 3) Heat stress time significantly increased the expression levels of HSP70 family genes (HSPA1A, HSPA6 and HSPA8) in blood lymphocytes. The mRNA expression of HSPA1A rose firstly, then declined, and then it reached the peak at 4 h after heat stress. In addition, the mRNA expression of HSPA1A was significantly higher at 2, 4, 8 and 12 h after heat stress than at 0 h (P<0.01). The mRNA expression of HSPA6 increased significantly at 2 h after heat stress (P<0.01), and returned to pre-stress level after 4 h (P>0.05). The mRNA expression of HSPA8 was significantly higher at 4 (P<0.05), 8 (P<0.01), 12 h (P<0.01) after heat stress than that at 0 h. In this experiment, acute heat stress at 38℃ could inhibit the immune and antioxidant functions and increased the mRNA expression of HSPA1A, HSPA6 and HSPA8 genes in blood lymphocytes. HSPA1A was more sensitive to temperature and time of heat stress, and could be used as a molecular marker of early heat stress in goats.

Key words: acute heat stress, goat, antioxidant capacity, immune function, heat shock protein 70 family genes

CLC Number:

S827.4

PENG Xiaokun, ZHAO Tian, HUANG Xiaoyu, ZHANG Yu, XING Xiaonan, ZHANG Enping. Effects of Acute Heat Stress on Blood Biochemistry Indices and Expression of HSP70 Family Genes in Blood Lymphocytes in Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1219-1229.

References

[1] GUPTA M,DANGI S S,MAURYA D,et al.Expression profile of cold shock protein genes in goats (Capra hircus) during different seasons[J].Iran J Vet Res,2014,15(1):7-12.
[2] APPLEMAN R D,DELOUCHE J C.Behavioral,physiological and biochemical responses of goats to temperature,0 to 40℃[J].J Anim Sci,1958,17(2):326-335.
[3] SEJIAN V,SRIVASTAVA R S.Effects of melatonin on adrenal cortical functions of Indian goats under thermal stress[J].Vet Med Int,2010,2010:348919.
[4] SALAMA A A K,CAJA G,HAMZAOUI S,et al.Different levels of response to heat stress in dairy goats[J].Small Rumin Res,2014,121(1):73-79.
[5] 张灿,王之盛,彭全辉,等.湿热应激对藏绵羊和山羊生长性能、抗氧化能力以及免疫功能的影响[J].动物营养学报,2017,29(6):2179-2187.
ZHANG C,WANG Z S,PENG Q H,et al.Effects of moist-heat stress on growth performance,oxidation resistance and immunity of Tibetan sheep and goats[J].Chinese Journal of Animal Nutrition,2017,29(6):2179-2187.(in Chinese)
[6] MAHJOUBI E,YAZDI M H,AGHAZIARATI N,et al.The effect of cyclical and severe heat stress on growth performance and metabolism in Afshari lambs[J].J Anim Sci,2015,93(4):1632-1640.
[7] HAMZAOUI S,SALAMA A A K,ALBANELL E,et al.Physiological responses and lactational performances of late-lactation dairy goats under heat stress conditions[J].J Dairy Sci,2013,96(10):6355-6365.
[8] POPOOLA M A,SAKA A A,OLANIYI T A,et al.Influence of temperature humidity index on skin temperature of West African dwarf goats raised in Nigeria[J].Agric Adv,2013,2(12):303-307.
[9] DARCAN N,CEDDEN F,CANKAYA S,et al.Spraying effects on some physiological and behavioural traits of goats in a subtropical climate[J].Italian J Anim Res,2008,7(1):77-85.
[10] NAGAYACH R,GUPTA U D,PRAKASH A.Expression profiling of hsp70 gene during different seasons in goats (Capra hircus),under sub-tropical humid climatic conditions[J].Small Rumin Res,2017,147:41-47.
[11] 蔡丽媛.集约化羊舍的环境控制及热应激对山羊瘤胃发酵的影响[D].武汉:华中农业大学,2015.
CAI L Y.Environmental control of intensive goat buildings and the effects of heat stress on rumen fermentation of goats[D].Wuhan:Huazhong Agricultural University,2015.(in Chinese)
[12] BAKTHISARAN R,TANGIRALA R,RAO C M.Small heat shock proteins:role in cellular functions and pathology[J].Biochim Biophys Acta,2015,1854(4):291-319.
[13] DEANE C A S,BROWN I R.Knockdown of heat shock proteins HSPA6(Hsp70B') and HSPA1A (Hsp70-1) sensitizes differentiated human neuronal cells to cellular stress[J].Neurochem Res,2018,43(2):340-350.
[14] DANGI S S,DANGI S K,CHOUHAN V S,et al.Modulatory effect of betaine on expression dynamics of HSPs during heat stress acclimation ingoat (Capra hircus)[J].Gene,2016,575(2):543-550.
[15] 唐雪峰,李建柱,赵存真,等.热休克蛋白70生物学功能及在家禽组织中的表达[J].畜牧与兽医,2018,50(4):144-148.
TANG X F,LI J Z,ZHAO C Z,et al.Biological function and expression of heat shock protein 70 in poultry tissues[J].Animal Husbandry & Veterinary Medicine,2018,50(4):144-148.(in Chinese)
[16] PARMAR M S,MADAN A K,HUOZHA R,et al.Heat shock protein70(HSP70) gene expression pattern in peripheral blood mononuclear cells (PBMCs) during different seasons in sahiwal cows (Bos Indicus)[J].J Anim Res,2015,5(1):109-113.
[17] DANGI S S,GUPTA M,NAGAR V,et al.Impact of short-term heat stress on physiological responses and expression profile of HSPs in Barbari goats[J].Int J Biometeorol,2014,58(10):2085-2093.
[18] BANERJEE D,UPADHYAY R C,CHAUDHARY U B,et al.Seasonal variation in expression pattern of genes under HSP70:seasonal variation in expression pattern of genes under HSP70 family in heat- and cold-adapted goats (Capra hircus)[J].Cell Stress Chaperones,2014,19(3):401-408.
[19] GUPTA M,KUMAR S,DANGI S S,et al.Physiological,biochemical and molecular responses to thermal stress in goats[J].Int J Livest Res,2013,3(2):27-38.
[20] CONG X,ZHANG Q,LI H T,et al.Puerarin ameliorates heat stress-induced oxidative damage and apoptosis in bovine Sertoli cells by suppressing ROS production and upregulating Hsp72 expression[J].Theriogenology,2017,88:215-227.
[21] 许啸.奶山羊高温预警指标的筛选及有机铬调控作用研究[D].武汉:华中农业大学,2013.
XU X.Sifting for the high temperature early warning indice in dairy goats and the regulating effect of organicchromium[D].Wuhan:Huazhong Agricultural University,2013.(in Chinese)
[22] 王立志.热应激对奶牛、奶山羊体内内毒素含量的影响及缓解热应激的营养技术研究[D].雅安:四川农业大学,2010.
WANG L Z.Effects of heat stress on endotoxin content in dairy cows and dairy goats and nutritional techniques for alleviating heat stress[D].Ya'an:Sichuan Agricultural University,2010.(in Chinese)
[23] 张轶凤,齐智利.热应激条件下机体发生氧化应激的机制[J].动物营养学报,2017,29(9):3051-3058.
ZHANG Y F,QI Z L.Mechanism of oxidative stress in body under heat stress[J].Chinese Journal of Animal Nutrition,2017,29(9):3051-3058.(in Chinese)
[24] YANG L,TAN G Y,FU Y Q,et al.Effects of acute heat stress and subsequent stress removal on function of hepatic mitochondrial respiration,ROS production and lipid peroxidation in broiler chickens[J].Comp Biochem Physiol C Toxicol Pharmacol,2010,151(2):204-208.
[25] PAMOK S,AENGWANICH W,KOMUTRIN T.Adaptation to oxidative stress and impact of chronic oxidative stress on immunity in heat-stressed broilers[J].J Therm Biol,2009,34(7):353-357.
[26] 魏凤仙,胡骁飞,张敏红,等.相对湿度和氨气应激对肉仔鸡血氨水平及细胞因子含量的影响[J].动物营养学报,2013,25(10):2246-2253.
WEI F X,HU X F,ZHANG M H,et al.Effects of relative humidity and ammonia stress on plasma ammonia level and cytokine contents of broilers[J].Chinese Journal of Animal Nutrition,2013,25(10):2246-2253.(in Chinese)
[27] YANG Z J,LIU C,ZHENG W J,et al.The functions of antioxidants and heat shock proteins are altered in the immune organs of selenium-deficient broiler chickens[J].Biol Trace Elem Res,2016,169(2):341-351.
[28] 于静,薛佳,胡艳欣,等.急性热应激对小鼠肺组织中热应激蛋白和细胞因子基因表达的影响[J].中国兽医杂志,2015,51(10):20-23.
YU J,XUE J,HU Y X,et al.The effects of acute heat stress on gene expression of several HSPs and cytokines in lung tissue of mice[J].Chinese Journal of Veterinary Medicine,2015,51(10):20-23.(in Chinese)
[29] 熊嫣.温热环境因子及氨气对肉鸡健康的影响机制研究[D].北京:中国农业科学院,2017.
XIONG Y.Effects of thermal environmental factors and ammonia on the broiler health[D].Beijing:Chinese Academy of Agricultural Sciences,2017.(in Chinese)
[30] 马燕芬,陈琦,杜瑞平,等.热应激对奶山羊瘤胃上皮细胞屏障通透性的影响[J].中国农业科学,2013,46(21):4478-4485.
MA Y F,CHEN Q,DU R P,et al.Effect of heat stress on rumen epithelial cell barrier permeability in dairy goat[J].Scientia Agricultura Sinica,2013,46(21):4478-4485.(in Chinese)
[31] CHEN J,ZHOU Y X,JIN X D,et al.Expression of interleukin-2 in Candidal balanoposthitis and its clinical significance[J].Chin Med J,2011,124(17):2776-2778.
[32] 韩爱云,左晓磊,姚清国,等.急性高温对肉鸡生理状态及细胞免疫的影响[J].江苏农业科学,2012,40(4):215-217.
HAN A Y,ZUO X L,YAO Q G,et al.Effects of acute high temperature on physiological status and cellular immunity in Broilers[J].Jiangsu Agricultural Sciences,2012,40(4):215-217.(in Chinese)
[33] 胡艳欣,佘锐萍,张洪玉,等.热应激后猪血清中IL-2、IFN-γ及TNF-α水平的动态变化[J].畜牧兽医学报,2006,37(5):496-499.
HU Y X,SHE R P,ZHANG H Y,et al.Studies on the dynamic changes of the level of IL-2,IFN-γ and TNF-α in porcine serum after heat stress[J].Acta Veterinaria et Zootechnica Sinica,2006,37(5):496-499.(in Chinese)
[34] 周瑞进,郭景茹,宿甲子,等.急性冷暴露对仔猪PBMC中HSP70和血浆抗炎性细胞因子IL-4、IL-10的影响[J].中国兽医学报,2010,30(5):677-680.
ZHOU R J,GUO H R,XU J Z,et al.Effect of acute cold exposure on the HSP70 expression of PBMC and the level of IL-4,IL-10 in plasma of piglets[J].Chinese Journal of Veterinary Science,2010,30(5):677-680.(in Chinese)
[35] OKAMURA M,LILLEHOJ H S,RAYBOURNE R B,et al.Cell-mediated immune responses to a killed Salmonella enteritidis vaccine:lymphocyte proliferation,T-cell changes and interleukin-6(IL-6),IL-1,IL-2,and IFN-γ production[J].Comp Immunol Microbiol Infect Dis,2004,27(4):255-272.
[36] 刘晓曦,刘凤华,刘明江,等.热应激大鼠肠道黏膜免疫及抗原递呈功能的变化[C]//中国畜牧兽医学会2013年学术年会论文集.北京:中国畜牧兽医学会,2013.
LIU X X,LIU F H,LIU M J,et al.Changes of heat stress on intestinal mucosal immunity and antigen presentation function in rats[C]//Proceedings of the 2013 Annual Academic Meeting of Chinese Association of Animal Science and Veterinary Medicine.Beijing:Chinese Association of Animal Science and Veterinary Medicine,CAAV,2013.(in Chinese)
[37] PELI A,SCAGLIARINI L,BERGAMINI P F,et al.Influence of heat stress on the immunity in growing beef cattle[J].Large Anim Rev,2013,19(5):215-218.
[38] 胡煜,蔡明成,王玲,等.热应激状态下牛血清生化指标、miRNA表达变化及其相关性分析[J].畜牧兽医学报,2016,47(9):1840-1847.
HU Y,CAI M C,WANG L,et al.The serum biochemical indexes and miRNA expression in cattle under heat stress and their correlation analysis[J].Acta Veterinaria et Zootechnica Sinica,2016,47(9):1840-1847.(in Chinese)
[39] MORINOBU A,KUMAGAI S.Cytokine measurement at a single-cell level to analyze human Th1 and Th2 cells[J].Rinsho Byori Jpn Clin Pathol,1998,46(9):908-1014.
[40] 刁华杰,冯京海,王雪洁,等.日循环高温对蛋鸡抗氧化能力及免疫机能的影响[J].畜牧兽医学报,2017,48(6):1044-1053.
DIAO H J,FENG J H,WANG X J,et al.Effect of cyclic high temperature on antioxidant capacity and immune function of laying hens[J].Acta Veterinaria et Zootechnica Sinica,2017,48(6):1044-1053.(in Chinese)
[41] 翟杰.热应激对雏鸡外周血液免疫细胞功能及相关因子的影响[D].哈尔滨:东北农业大学,2015.
ZHAI J.The effect of heat stress on the function of immunologic cell and cytocines in peripheral blood of chicks[D].Harbin:Northeast Agricultural University,2015.(in Chinese)
[42] WU Y N,YAN F F,HU J Y,et al.The effect of chronic ammonia exposure on acute-phase proteins,immunoglobulin,and cytokines in laying hens[J].Poult Sci,2017,96(6):1524-1530.
[43] ARCHANA P R,ALEENA J,PRAGNA P,et al.Role of heat shock proteins in livestock adaptation to heat stress[J].J Dairy Vet Anim Res,2017,5(1):00127.
[44] LIEW P K,ZULKIFLI I,HAIR-BEJO M,et al.Effects of early age feed restriction and heat conditioning on heat shock protein 70 expression,resistance to infectious bursal disease,and growth in male broiler chickens subjected to heat stress[J].Poult Sci,2003,82(12):1879-1885.
[45] COLLIER R J,COLLIER J L,RHOADS R P,et al.Invited review:genes involved in the bovine heat stress response[J].J Dairy Sci,2008,91(2):445-454.
[46] NOONAN E J,PLACE R F,GIARDINA C,et al.Hsp70B' regulation and function[J].Cell Stress Chaperones,2007,12(3):219-229.
[47] LEUNG T K,RAJENDRAN M Y,MONFRIES C,et al.The human heat-shock protein family.Expression of a novel heat-inducible HSP70(HSP70B') and isolation of its cDNA and genomic DNA[J].Biochem J,1990,267(1):125-132.
[48] ROHDE M,DAUGAARD M,JENSEN M H,et al.Members of the heat-shock protein 70 family promote cancer cell growth by distinct mechanisms[J].Genes Dev,2005,19(5):570-582.
[49] SONNA L A,WENGER C B,FLINN S,et al.Exertional heat injury and gene expression changes:a DNA microarray analysis study[J].J Appl Physiol,2004,96(5):1943-1953.
[50] 白丹丹,敖日格乐,王纯洁,等.慢性冷热应激对三河牛血液生化指标及相关基因表达的影响[J].中国农业大学学报,2017,22(8):50-56.
BAI D D,AORIGELE,WANG C J,et al.Influences of chronic cold and heat stress on blood biochemical parameters and related gene expression in Sanhe cattle[J].Journal of China Agricultural University,2017,22(8):50-56.(in Chinese)
[51] HOOPER H B,DOS SANTOS SILVA P,DE OLIVEIRA S A.et al.Acute heat stress induces changes in physiological and cellular responses in Saanen goats[J].Int J Biometeorol,2018,62(12):2257-2265.
[52] 高雅君.炎症条件下草鱼HSP70的表达及HSF1对其调控的初步研究[D].成都:电子科技大学,2018.
GAO Y J.Preliminary study on the expression of HSP70 and its regulation by HSF1 under inflammatory conditions in grass carp[D].Chengdu:University of Electronic Science and Technology of China,2018.(in Chinese)
[53] 肖定福,张彬,胡雄贵.热应激蛋白对畜禽抗应激机理的研究进展[J].中国畜牧兽医,2009,36(4):58-62.
XIAO D F,ZHANG B,HU X G.Research progress on anti-stress mechanism of HSPs to livestock and poultry[J].China Animal Husbandry & Veterinary Medicine,2009,36(4):58-62.(in Chinese)
[54] GAUBIN Y,VAISSADE F,CROUTE F,et al.Implication of free radicals and glutathione in the mechanism of cadmium-induced expression of stress proteins in the A549 human lung cell-line[J].Biochim Biophys Acta,2000,1495(1):4-13.
[55] SHIMIZU M,TAMAMORI-ADACHI M,ARAI H,et al.Lipopolysaccharide pretreatment attenuates myocardial infarct size:a possible mechanism involving heat shock protein 70-inhibitory κBα complex and attenuation of nuclear factor κB[J].J Thorac Cardiovasc Surg,2002,124(5):933-941.
[56] ASEA A,KRAEFT S K,KURT-JONES E A,et al.HSP70 stimulates cytokine production through a CD14-dependant pathway,demonstrating its dual role as a chaperone and cytokine[J].Nat Med,2000,6(4):435-442.
[57] MILLAR D G,GARZA K M,ODERMATT B,et al.Hsp70 promotes antigen-presenting cell function and converts T-cell tolerance to autoimmunity in vivo[J].Nat Med,2003,9(12):1469-1476.
[58] ROUT P K,KAUSHIK R,RAMACHANDRAN N.Differential expression pattern of heat shock protein 70 gene in tissues and heat stress phenotypes in goats during peak heat stress period[J].Cell Stress Chaperones,2016,21(4):645-651.

Effects of Acute Heat Stress on Blood Biochemistry Indices and Expression of HSP70 Family Genes in Blood Lymphocytes in Goats

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