禽致病性大肠杆菌双组分系统phoP/Q对鞭毛Ⅲ型分泌系统基因的调控预测

doi:10.11843/j.issn.0366-6964.2019.04.015

Abstract

Abstract:

The purpose of this study was to investigate the relationship between the two-component system phoP/Q and flagella type Ⅲ secretory system (flagellar T3SS) proteins in Avian Pathogenic Escherichia coli (APEC). We used software to analyze the function and to predict the relationship between phoP/Q and flagella T3SS, and used gene chip technology to compare the transcriptome differences between APEC wild strain and ΔphoP/Q deletion mutant, and screening for variable genes in flagellum T3SS pedestal. The changes of gene expression were detected by real-time PCR. The results predicted by STRING showed that phoP/Q may regulate flagellar T3SS indirectly by regulating the two-component system near the flagellar T3SS locus. Gene chip screening and real-time PCR revealed that the phoP/Q deletion could significantly differ the expression of most genes in the core component of flagellar T3SS, and also affect the expression of motA, motB and cheY in the two components. In this study, the relationship between phoP/Q two-component system and flagellar T3SS was predicted and explored, which provided a new breakthrough point and research direction for further research on bacterial activity and pathogenic mechanism.

Key words: avian pathogenic Escherichia coli, phoP/Q, flagellar T3SS, gene chip

CLC Number:

S852.612

DENG Zhiwen, WANG Zeping, LI Qianwen, YIN Lei, TU Jian, SONG Xiangjun, QI Kezong. Prediction of the Regulation of Flagell in Type Ⅲ Secretion System Genes by Two-component System phoP/Q in Avian Pathogenic Escherichia coli[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 821-829.

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Prediction of the Regulation of Flagell in Type Ⅲ Secretion System Genes by Two-component System phoP/Q in Avian Pathogenic Escherichia coli

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[1]	DENG Zhiwen, WANG Zeping, LI Qianwen, YIN Lei, TU Jian, SONG Xiangjun, QI Kezong. Prediction of the Regulation of Flagell in Type Ⅲ Secretion System Genes by Two-component System phoP/Q in Avian Pathogenic Escherichia coli [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 821-829.
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