塞内卡病毒A结构蛋白VP1诱导PK-15细胞凋亡

doi:10.11843/j.issn.0366-6964.2019.04.014

Abstract

Abstract:

As a new pathogen, the pathogenesis of Senecavirus A (SVA) remains unclear. Through microscopic observation, it was found that SVA can infect PK-15 cells and causes significant cytopathic (CPE). Concomitant with CPE, apoptosis is commonly observed in infected cells. In this study, the function of each individual SVA protein in the induction of apoptosis was studied. Firstly, using Western blotting confirmed the successful expression of SVA proteins. Subsequently, the SVA proteins were screened for their apoptotic function using flow cytometry with Annexin V-FITC/PI double staining. Then, the extent of externalized phospholipid phosphatidylserine and nuclear condensation induced by VP1 was analyzed by fluorescence microscope using Annexin V-FITC/PI and Hoechst staining. Finally, Western blotting and RT-PCR technique were used to analyze the effects of VP1 on the protein and mRNA levels of the major apoptosis regulatory molecules Caspase3, Caspase8 and Bax. SVA-VP1 was found to significantly induce early and late apoptosis in cells. And it also could promote the up-regulation of pro-apoptotic protein Caspase3, Caspase8 and Bax protein and mRNA levels. This study provides a theoretical basis for further research in molecular mechanism and the pathogenesis of SVA-regulated apoptosis.

Key words: Senecavirus A, PK-15 cells, apoptosis, flow cytometry

CLC Number:

S852.659.6

WANG Yong, MAO Ruoqing, ZHANG Keshan, ZHENG Haixue, LIU Xiangtao. The Structural Protein of Senecavirus A VP1 Induces Apoptosis in PK-15[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 811-820.

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The Structural Protein of Senecavirus A VP1 Induces Apoptosis in PK-15

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