产肠毒素大肠杆菌感染IPEC-J2细胞后IL-17细胞因子表达变化及其功能初探

doi:10.11843/j.issn.0366-6964.2019.04.016

Abstract

Abstract:

Enterotoxigenic E. coli (ETEC) is one of the most important cause of neonatal and post-weaning diarrhea (PWD) in piglets. The interaction between ETEC and intestinal epithelial cells is the prerequisite of bacterial pathogenesis, as well as the first step of triggering host immune response. The aim of this study was to determine the expression profile and host defense mechanisms of IL-17 cytokine in porcine intestinal epithelial cells against ETEC infection. Firstly, we determined the expression of IL-17 cytokines and several mucosal defense genes by the application of RT-PCR, ELISA, immunohistochemistry (IHC) assays and Western Blotting in the porcine intestinal epithelial cell line IPEC-J2 infected with a F4⁺ETEC reference strain C83901. Later on, the regulating effect of IL-17 cytokine on the expression of above mucosal defense genes in IPEC-J2 cells was explored. The results showed that C83901 induced the mRNA expression of all IL-17 cytokines (IL-17D was not detected), particularly upregulated the expression of IL-17A and IL-17C both at the transcriptional and protein level. The upregulation of IL-17A and IL-17C in the protein level were confirmed by IHC and ELISA. The existence of IL-17A and/or IL-17C either induced by ETEC infection or supplemented artificially in IPEC-J2 cells can elicit the expression of host defense gene expression of mucin-2, claudin-1, claudin-2 and beta defensins-2. These results indicated that IL-17 induction in intestinal epithelial cells upon ETEC infection are beneficial for the mucosal host defense by promoting the intestinal barrier integrity.

Key words: interleukin-17, enterotoxigenic E. coli, intestinal epithelial cells, innate immune response, host defense

CLC Number:

S852.612

LUO Yu, XU Jia, ZHANG Chaoying, JIANG Chunyan, HE Haijian, YU Jianguo, ZHANG Hongbing. The Expression and Functional Analysis of IL-17 Cytokine Family in Enterotoxigenic E.coli Infected IPEC-J2 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(4): 830-839.

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The Expression and Functional Analysis of IL-17 Cytokine Family in Enterotoxigenic E.coli Infected IPEC-J2 Cells

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