牦牛鲜精与冻精HSP70表达差异比较研究

doi:10.11843/j.issn.0366-6964.2018.11.024

Abstract

Abstract:

The aims of this study were to investigate changes of sperm quality and heat shock protein(HSP)70 expression before and after freezing yak semen. The semen of 6 healthy Datong yaks with normal reproductive ability was collected and assigned to 2 groups:fresh group and frozen-thawed group. The quality of sperm was evaluated by assessing sperm motility and plasma membrane integrity. The expression of HSP70 in both groups was detected by the real-time quantitative polymerase chain reaction,Western blot and immunofluorescence techniques. The results showed that,compared with fresh sperm,sperm motility and plasma membrane integrity of frozen-thawed sperm were significantly decreased (P<0.01); In addition,the expression of HSP70 was significantly lower at both the transcriptional (P<0.05) and protein (P<0.01) levels. HSP70 protein was distributed over the sperm acrosome,acrosome equatorial segment,post-acrosomal and middle sections of sperm in the fresh group. In contrast,after freezing and thawing,HSP70 protein was mainly located in post-acrosomal and middle sections of sperm,and the average optical density was significantly decreased(P<0.01). In conclusion,yak sperm quality and the expression of HSP70 declined simultaneously after freezing and thawing,suggesting that they may be associated.

Key words: HSP70, yak, sperm motility, plasma membrane integrity, immunofluorescent

CLC Number:

S823.8⁺5.2

TANG Peng, LING Xiao-xiao, JIA Cong-jun, LIANG Chun-nian, WU Xiao-yun, WANG Hong-bo, CHU Min, YAN Ping. Study on the Differences of HSP70 Expression in Fresh Sperm and Frozen-thawed Sperm in Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2514-2520.

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Study on the Differences of HSP70 Expression in Fresh Sperm and Frozen-thawed Sperm in Yak

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