ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2018, Vol. 49 ›› Issue (11): 2505-2513.doi: 10.11843/j.issn.0366-6964.2018.11.023

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Effects of TRPV6 Gene Silencing on the Expression of Proteins Involved in Calcium Ions Transcellular Transport in Duodenum and Jejunum of Laying Hens

MENG Fan-yong1, DENG Yi-feng1, YANG Jun-hua2, CUI Jun1, ZHOU Zhen-lei1, HOU Jia-fa1*   

  1. 1. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China;
    2. Institute for Agri-food Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
  • Received:2018-03-12 Online:2018-11-23 Published:2019-01-23

Abstract:

This study was conducted to investigate the effect of transient receptor potential vanilloid receptor 6 (TRPV6) gene silencing on expression of proteins involved in calcium ions transcellular transport in duodenum and jejunum, concentration of calcium and phosphorus and PTH level in plasma, and bone mineral density of femur and tibia in laying hens. Sixty-four laying hens at the peak stage (220 days old) were assigned to control and TRPV6 gene silencing groups, and injected, respectively with physiological saline or interference vector pSIREN-TRPV6-3 in one time, and continuously observed for 28 d. The results were as follows:compared with control group, the expression of ZsGreen protein as an indicator of transfection in duodenum and jejunum were successfully detected at the 7th, 14th and 21st day after injection. The mRNA and protein expression of TRPV6 and CaBP-D28K in duodenum and jejunum exposed to TRPV6 gene silencing were observably decreased at the 7th, 14th and 21st day (P<0.05 or P<0.01). Additionally, the level of PTH in plasma was markedly increased compared with control (P<0.05 or P<0.01). However, the concentration of calcium and phosphorus in plasma were not affected by injection of interference vector pSIREN-TRPV6-3, and the bone mineral density of femur and tibia in the group of TRPV6 gene silencing were slightly lower than control group (P>0.05). It was concluded that the transfection of pSIREN-TRPV6-3 plasmid could suppress the expression of TRPV6 and CaBP-D28K in duodenum and jejunum, but doesn't regulate the concentration of calcium in plasma and bone mineral density, it is implied that silence of TRPV6 gene does not affect normal calcium transport of laying hens under the condition of normal calcium content.

Key words: TRPV6, RNAi, laying hens, intestine, calcium ion transport

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