禽白血病病毒在惠阳胡须鸡和汶上芦花鸡的垂直传播特性分析

doi:10.11843/j.issn.0366-6964.2018.07.017

Abstract

Abstract:

This experiment was conducted to find the vertical transmission rule of avian leukosis virus(ALV) in indigenous chickens. Huiyang chicken and Wenshang Barred chicken were selected as materials,two experimental groups were set up based on virus isolation detection results of plasma and semen at the age of 43 weeks and pedigree analysis:group Ⅰ,male and female parent were ALV-positive Huiyang chicken and ALV-negative Wenshang Barred chicken which was divided into three groups and eight chicken in each group;group Ⅱ,male and female parent were ALV-negative Wenshang Barred chicken and ALV-positive Huiyang chicken.The hybrid offsprings were selected and raised separately,the meconium,cloacal swabs and plasma were collected at 1, 42, 42 days of age for p27 antigen detection and virus isolation detection. The subtype of ALV-positive chicken through virus isolation were verified by PCR,sequencing and phylogenetic analysis. The results showed as follows:Positive rates of p27 antigen from meconium, cloacal swabs and virus isolation in offsprings of ALV-negative roosters were significantly higher than those in offsprings of ALV-positive hens(P<0.05). The virus isolation rate in offsprings of ALV-negative roosters and ALV-positive hens were 8.54% and 0%,respectively. There was no significant correlation between amount of ALV carried or shed by ALV-positive roosters and hens and positive rate of p27 antigen in their offsprings(P>0.05). The false positive rate of p27 antigen from meconium and cloacal swabs was high, and misdetection rate of p27 antigen from meconium was 15% compared to virus isolation. Sequencing and phylogenetic analysis showed that ALV-positive chicken through virus isolation were all ALV-J. This study suggest that roosters would play an important role in vertical transmission of ALV,and it would be necessary to strengthen the surveillance of roosters which were infected with ALV in eradication process of avian leuiosis.

Key words: avian leukosis, vertical transmission, indigenous chicken

CLC Number:

S852.659.3

ZHANG Hui-yong, WU Jing-sheng, LI Guo-hui, YU Yan, YANG Jian-bo, SU Yi-jun, YIN Jian-mei, HAN Wei. Characteristic Analysis on Vertical Transmission of Avian Leukosis Virus in Huiyang Chicken and Wenshang Barred Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1475-1481.

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Characteristic Analysis on Vertical Transmission of Avian Leukosis Virus in Huiyang Chicken and Wenshang Barred Chicken

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