猪繁殖与呼吸综合征弱毒活疫苗免疫猪脾脏转移因子的制备及其活性分析

doi:10.11843/j.issn.0366-6964.2018.07.016

Abstract

Abstract:

The objective of this study was to prepare transfer factor(TF) from the spleen of pig immunized with porcine reproductive and respiratory syndrome(PRRS) attenuated-live virus vaccine, then detect its immune activities and expect to provide a scientific basis for developing a new immunopotentiator and prevention and control of PRRS. TF was extracted from spleen collected from healthy grown pigs which had been vaccinated with PRRS attenuated-live virus vaccine. The main components of TF were detected using sulfonyl salicylic acid, ninhydrin and ammonium molybdate-ammonium nitrate reactions, and kinds and contents of amino acids were measured by high performance liquid chromatography. The regulating effects on phagocytosis of peritoneal macrophage and lymphocytic proliferation of mice were checked by chicken erythrocyte phagocytosis test and MTT assay, respectively. Furthermore, nine piglets were divided into group A, B and C of 3 each. The piglets in the group A were injected intramuscularly with one dose of PRRS attenuated-live virus vaccine in left side opisthotic part and 1 mL of TF in the right side, respectively. Each one in the group B was injected intramuscularly the mixed liquor of one dose of PRRS attenuated-live virus vaccine with 1 mL of TF, and in the group C was did the mixed liquor of one dose of PRRS attenuated-live virus vaccine with 1 mL of normal saline. The proliferative activity of porcine peripheral blood lymphocyte(PBLC) from each group was measured by MTT on day 20 post-inoculation. There were nucleic acid and 18 kinds of amino acids in TF prepared in this study, containing 21.5 μg of nucleic acid and 3.479 mg total amino acids per mL of TF. Phagocytic ability of peritoneal macrophage to chicken erythrocytes and proliferative activity of PBLC from mice were enhanced significantly following stimulation of TF. Moreover, the proliferation capacity of porcine PBLC was also increased remarkably when the four-week-old piglets were injected with PRRS attenuated-live virus vaccine and TF simultaneously in different parts or their mixture. The transfer factor was prepared from the spleen of pig immunized with PRRS attenuated-live virus vaccine and it could increase significantly phagocytosis of macrophage and proliferative activity of PBLC.

Key words: PRRSV, pig, spleen, transfer factor, immunological activities

CLC Number:

S852.659.6

GUO Hai-yong, YUAN Hong-xing, SUN Tai-ran, CAO Jing, SONG Qin-ye, LI Tan-qing, LU Ji-cheng. Preparation and Immunological Activities of Transfer Factor from the Spleen of Pig Immunized with Porcine Reproductive and Respiratory Syndrome Attenuated-live Virus Vaccine[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(7): 1467-1474.

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Preparation and Immunological Activities of Transfer Factor from the Spleen of Pig Immunized with Porcine Reproductive and Respiratory Syndrome Attenuated-live Virus Vaccine

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