一株新亚群禽白血病病毒全基因组序列分析

doi:10.11843/j.issn.0366-6964.2017.09.017

Abstract

Abstract:

In order to understand the epidemic situation of exogenous avian leukemia virus (ALV) in broiler flocks in Shandong province, blood plasma were collected for virus isolation and identification by ELISA (detection of P27 antigen) and PCR. A strain of new ALV subgroup was isolated and identified from commercial broiler flocks in a farm of Shandong province, named FC1505. The env gene of the isolate was sequenced and indicated that the amino acid sequence of gp85 had the highest gp85 identity of more than 95% to the strains of suspected subgroup K of ALV from Chinese native breed chickens. Its gp85 identity to other known chicken ALV subgroups A-E and J was lower than 90%. In order to further analyze the molecular properties of the isolate, the genome of the virus was sequenced and compared with those from other ALV reference strain of different subgroups. The results showed that the gag, pol and gp37 genes of the isolate were relatively conservative, with the identity more than 90%, and showed the highest identity to suspected ALV-K isolates. Whole genome sequence analysis supported that the FC1505 isolate belongs to ALV-K. Following the reports of K subgroup ALV in Jiangsu and Southern China provinces of China, this is the first report of an ALV-K isolate from broilers of Shandong province and its whole genome analysis.

CLC Number:

S852.659.3

LI Jiu-qing, LIU Qiang, GUO Lei, XU Chuan-tian, CUI Ning, LI Bing, CUI Zhi-zhong. Genomic Sequence Analysis of a New Subgroup of Avian Leukosis Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(9): 1718-1723.

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Genomic Sequence Analysis of a New Subgroup of Avian Leukosis Virus

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