种蛋中内源性禽白血病病毒的检测和鉴定

doi:10.11843/j.issn.0366-6964.2014.08.016

Abstract

Abstract:

A breeder chicken flock demonstrated 5%-6% positive rates in tests of antibodies to subgroups A/B avian leukosis virus (ALV) and egg albumen p27 antigen，although chickens were clinically health and their egg productivity was normal.However，no exogenous ALV was isolated when serum and egg albumen samples of more than 200 birds were inoculated in DF1 cell cultures.Then 23 breeders with high S/P values in ELISA for p27 antigen in their egg albumen were selected and kept from the breeder farms.Blood plasma and egg albumen samples were collected twice from each birds for inoculation of DF1 cells and SPF embryo-originated fibroblast (CEF)cultures，again there was no exogenous ALV isolated，in order to identify whether the virus is endogenous viruses.Fertilized eggs from these 23 breeders were incubated for 9-11 d and their CEF were prepared for each embryos，p27 antigen was detected in CEF supernatant from only one of the 23 breeder eggs.Its supernatant was further inoculated into DF1 and SPF embryo CEF culture，p27 was detected from only SPF embryo CEF but not DF1 cell cultures，indicating that there was endogenous but not exogenous ALV.By use of genomic RNA abstracted from viral particles in the supernatant with p27 detected as the template，gp85 gene was amplified by RT-PCR.Sequence comparison confirmed that the detected ALV belonged to subgroup E.After immunization with its gp85 protein expressed in E.coli，some chickens demonstrated antibody positive by ALV-A/B antibody detection ELISA kit.Influence or interference of ALV-E on results in tests with ALV antibody or p27 detection ELISA kits was discussed.

CLC Number:

S852.659.3

XU Hai-peng，MENG Fan-feng，DONG Xuan，ZHAO Peng，CUI Zhi-zhong. Detection and Identification of an Endogenous Subgroup E Avian Leukosis Virus in a Chicken Breeder Embryo[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1317-1323.

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Detection and Identification of an Endogenous Subgroup E Avian Leukosis Virus in a Chicken Breeder Embryo

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