乳酸链球菌素对大肠杆菌所致腹泻小鼠的脑-肠轴中单胺类神经递质的影响

doi:10.11843/j.issn.0366-6964.2016.09.023

Abstract

Abstract:

The paper aims to study the correlation between Streptococcus thermophilus and intestinal flora and brain-gut axis function during diarrhea irritable，and further reveals the regulation mechanism of nisin on monoamine neurotransmitters.By injecting three serotypes of Escherichia coli mixed bacterium suspension liquid (1∶1，1.0×109 CFU• mL－1),the diarrhea model of mice was prepared，and nisin and Mongolian medicine were used for treatment.Tissus samples were collected at 0 h，72 h post modeling and ELISA kits were used to detect the levels of 5-HT，DA and NE，as well as the caecum contents plate were diluted 10 fold into eight gradient coating on the responging medium to calculate bacterial colony number by using plat coating method.The results showed that：(1)In brain tissue,5-HT level at 72 h post modeling in the nisin，traditional Mongolian medicine（TMM） compound，TMM ingredients，and antibiotic groups were increased to 108.60%，124.04%，117.47%，115.57% of 0 h， respectively; and the duodenal 5-HT in the nisin， TMM compound and TMM ingredients groups at 72 h post modeling were increased to 289.18%，200.30%，271.59% of 0 h,respectively，nisin group was significantly higher than other groups (P<0.05).After modeling，in addition to the jejunum，compared 72 h’s with 0 h’s，nisin and TMM compound have signicantly improved 5-HT level （P<0.05） in mice serum，brain，duodenum，ileum and colon.(2) After 72 h，nisin，TMM compound，TMM ingredients and antibiotic can improve the dopamine in the intestine and brain of normal mice,and that in the ileum increased the most，and the difference was significant (P<0.05).(3)In brain tissue at 0 h and 72 h, NE concentration increased to 115.62% and 106.17%，respectively under the react of TMM compound and nisin,and which also have significant difference comparing with blank control，and the negative control group (P<0.05)；in the duodenum and ileum，the EN concentration of nisin group at by 72 h were increased by 170.97%，121.74%，especially that in the duodenum was higher than other groups and the difference was significant (P<0.05).(4) Nisin，TMM effective ingredients and TMM compound can significantly increase the number of intestinal probiotic Lactobacillus and Bifidobacterium in E.coli infected mice，and significantly reduce the number of harmful bacteria Enterobacter and Enterococcus (P<0.05).In addition，nisin and TMM compound were significantly increased bacterial infections in mice blood，brain and various intestinal segments in 5-HT，NE content，especially in the duodenum，nisin and traditional Mongolian medicine can break the balance of intestinal 5-HT，and accompanied by increase of 5-HT in the intestine and blood,the brain 5-HT content also increased.

Key words: nisin, Mongolian medicine, neurotransmitter, stress-diarrhea, intestinal flora

CLC Number:

S852

JIA Zhi-feng，WANG Chun-jie，AO-Ri-ge-le，GAO Rui-juan，BA Sen-hu. Effect of Nisin on Monoamine Neurotransmitters of Mice with Diarrhea Induced by Escherichia coli[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1931-1939.

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Effect of Nisin on Monoamine Neurotransmitters of Mice with Diarrhea Induced by Escherichia coli

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