脱氧雪腐镰刀菌烯醇暴露对断奶仔猪延髓脂质过氧化反应、神经递质及钙稳态的影响

doi:10.11843/j.issn.0366-6964.2019.05.020

Abstract

Abstract:

This experiment was conducted to investigate the nerve toxicological effects of medulla oblongata tissues induced by deoxynivalenol (DON), through the changes of lipid peroxidation, neurotransmitter secretion and calcium homeostasis. Thirty weaned piglets (21 days old) were randomly divided into control group, low-dose and high-dose DON groups, with 10 piglets in each group. The piglets in control group were fed with basal diet, and DON low-dose group and high-dose group were fed with DON diet containing 1 and 2 mg·kg^-1 DON, respectively. The trial was conducted for 60 days. At the end of the experiment, five piglets were randomly selected from each group for slaughter. The medulla oblongata tissue was collected to detect the activities of oxidation and antioxidation indexes, neurotransmitter and Ca²⁺ concentrations, the relative expression of calmodulin (CaM) and calmodulin-dependent kinase Ⅱ (CaMK Ⅱ) mRNA, and the protein expression levels of CaM, CaMKⅡ and p-CaMKⅡ were measured. Compared with the control group, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in medulla oblongata of piglets in high and low doses of DON group decreased significantly (P<0.01), while the concentration of nitric oxide (NO) increased significantly (P<0.01). The concentration of serotonin (5-HT) in high and low doses of DON group were significantly increased (P<0.01),while the concentration of acetylcholine (ACH) was significantly decreased (P<0.01). The concentration of dopamine (DA) and gamma-aminobutyric acid (GABA) in high doses of DON group were significantly decreased (P<0.05).The concentrations of Ca²⁺ in high and low doses of DON group were significantly increased (P<0.01).The relative expression of CaM mRNA in low doses of DON group were significantly decreased (P<0.05), the expression of CaMKⅡ mRNA in high and low doses of DON group were significantly decreased (P<0.01),the expression level of CaM protein in high doses of DON group was significantly decreased (P<0.01), while the expression of p-CaMKⅡ protein was significantly increased (P<0.01). These results suggest that DON exposure can change the lipid peroxidation reaction and the secretions of neurotransmitters and neuronal calcium homeostasis in medulla oblongata tissues of weaned piglets. It suggests that DON has some neurotoxic effects in piglets.

CLC Number:

S856.9

CHU Xiaoyan, ZHU Lei, CAO Li, CHEN Xiaofang, LI Yu, FENG Shibin, WU Jinjie, WANG Xichun. Effects of Deoxynivalenol Exposure on Lipid Peroxidation, Neurotransmitters and Calcium Homeostasis in the Medulla Oblongata of Weaned Piglets[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1091-1098.

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Effects of Deoxynivalenol Exposure on Lipid Peroxidation, Neurotransmitters and Calcium Homeostasis in the Medulla Oblongata of Weaned Piglets

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