恩诺沙星灌注液在健康母猪体内的药代动力学研究

doi:10.11843/j.issn.0366-6964.2016.07.024

Abstract

Abstract:

This study was designed to compare the pharmacokinetic parameters of enrofloxacin following intravenous and intrauterine administrations to investigate their bioavailability in sows.16 cross-bred (Landrace×Yorkshire) healthy，adult，non-lactating sows，were used.The sows were randomly divided into two groups with eight animals each.The sows of first group were administered with enrofloxacin injection by intravenous route，at a single dose of 2.5 mg•kg^-1；whereas those of second group were administered with enrofloxacin uterine perfusate by intrauterine administration at a single dose of 50 mL per sow.Enrofloxacin concentrations were determined by reverse phase-high performance liquid chromatography (RP-HPLC) with fluorescence detection.A non-compartmental analysis of drug plasma concentration vs.time profiles was performed with pharmacokinetic modeling using Winnonlin software.Following intravenous administration，the main pharmacokinetic parameters were as follows：t _1/2（21.71±1.66）h，Vd（1.89±0.33）L•kg^-1， Cl（60.18±8.92）mL•h^-1•kg^-1，AUC_0-t（39.94±5.75）mg•h•L^-1，AUC_0-∞（42.32±6.03）mg•h•L^-1.The absorption of enrofloxacin was rapid and well absorbed after intrauterine administration，with t _1/2 of （28.55±2.31）h，T_maxof （3.19±1.46）h，C_max of （1.61±0.20）mg•L^-1，AUC_0-t of （49.06±5.18）mg•h•L^-1，AUC _0-∞（53.55±5.84）mg•h•L^-1.The absolute bioavailability (F) was 63.15%.Concentrations of the active metabolite ciprofloxacin were not detected in any samples.The results showed that the sows can absorb enrofloxacin well by way of intrauterine administration，with slow elimination and high bioavailability.And the effect on body after intrauterine administration should be considered.

Key words: enrofloxacin, uterine perfusate, pharmacokinetics, sows

CLC Number:

S859.7

FENG Ting-song，HUANG Xian-hui，ZENG Song-qing，GAO Jin，XIE Shun，LIU Yu.

Pharmacokinetics of Enrofloxacin Perfusate in Healthy Sows[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1488-1494.

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Pharmacokinetics of Enrofloxacin Perfusate in Healthy Sows

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