胰高血糖素样肽-2对脂多糖应激的IPEC-J2细胞形态和紧密连接相关基因表达的影响

doi:10.11843/j.issn.0366-6964.2015.04.012

Abstract

Abstract:

The purpose of this research was to investigate the effect of glucagon-like peptide-2(GLP-2) and lipopolysaccharide (LPS) on the gene expression of tight junction in piglets jejunum epithelial IPEC-J2 cells and to deeply discuss the possible mechanism of GLP-2 regulating the expression of intestinal TJ gene of the piglet.Trial 1，single factor design was adopted and 4 treatments(control，GLP-2，LPS，LPS+GLP-2) were used to test the effect of GLP-2 and LPS on the mRNA expression of Occludin，Claudin-1 and ZO-1 in IPEC-J2 cells.The results showed that：100 nmol•L^-1 GLP-2 could improve cellular morphology and significantly increase the expression level of Occludin，Claudin-1 and ZO-1 mRNA in IPEC-J2 cell (P<0.01)；100 μg•mL^-1 LPS could significantly destroy cellular morphology and significantly reduce the mRNA expression of TJ in IPEC-J2 cells (P<0.01).LPS with 100 nmol•L^-1 GLP-2 could improve cellular morphology and significantly increase the expression of Occludin，Claudin-1 and ZO-1 mRNA in IPEC-J2 cell (P<0.01) for 46.3%，65.1% and 30.3%，respectively.Trial 2，PI3K specific inhibitor，Wortmannin (Wort) and LY294002 (LY)，were added to investigate whether GLP-2 modulates TJ’s mRNA expression in IPEC-J2 cells through PI3K-Akt-mTOR signal transduction pathway.Four treatments(control，GLP-2，GLP-2+Wort，GLP-2+LY) were designed.The results showed that：100 nmol•L^-1 GLP-2 with 10 nmol•L^-1 Wort could significantly decrease the expression of Akt，mTOR，Occludin，Claudin-1 and ZO-1 mRNA in IPEC-J2 cells (P<0.01) for 46.9%，50.5%，38.1%，49.6% and 18.9%，respectively；GLP-2 with LY could significantly decrease the expression of Akt，mTOR，Occludin，Claudin-1 and ZO-1 mRNA in IPEC-J2 cells (P<0.01) for 67.2%，70.8%，49.6%，60.9% and 25.8%，respectively.In summary，the results showed that GLP-2 can effectively inhibit the damnification of TJ mRNA expression by LPS.GLP-2 may modulate TJ’s mRNA expression through PI3K-Akt-mTOR signal transduction pathway in IPEC-J2 cells.

Key words: glucagon-like peptide-2, lipopolysaccharide, piglet intestinal epithelial cell jejunum 2, tight junctions, PI3K-Akt-mTOR

CLC Number:

S828
S815

YU Chang-song，JIA Gang，DENG Qiu-hong，CHEN Xiao-ling，ZHAO Hua，LIU Guang-mang，WANG Kang-ning. The Effects of GLP-2 on Cell Morphology and the Gene Expression of Tight Junction in LPS Stressed IPEC-J2 Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 592-599.

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The Effects of GLP-2 on Cell Morphology and the Gene Expression of Tight Junction in LPS Stressed IPEC-J2 Cells

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