线粒体移植对水牛卵母细胞发育潜能的影响

doi:10.11843/j.issn.0366-6964.2015.04.011

Abstract

Abstract:

The aim of present study was to investigate the effects of mitochondrial transplant (MIT) on the developmental potential of buffalo oocytes.The mitochondrial DNA (mtDNA) copy number in the oocyte，and the developmental competence，exogenous mitochondria distribution，as well as mitochondrial membrane potential (ΔΨm) in the preimplantation embryos after the MIT were examined.The result showed that the average mtDNA copy number of buffalo oocytes in the first level of oocytes was evidently higher than the second and the third level of oocytes (（202 101±74 432） vs （118 483±17 028），（39 177±7 938），P＜0.01），and the average mtDNA copy number in the second level of oocytes was significantly higher than that of the third level of oocytes (（118 483±17 028） vs （39 177±7 938），P<0.01).Furthermore，the cleavage rates and blastocyst rates of the first level of oocytes were significantly higher than those of the second and the third level of oocytes after parthenogenetic activation (P<0.01)，and the cleavage rates and blastocyst rates of the second level of oocytes were remarkably higher than those of the third level of oocytes (P<0.01).The exogenetic mitochondria labeled with Mito-Tracker fluorescent probe distributed into each of blastomeres with the embryonic development after MIT.Moreover，more the second level of oocytes that received MIT developed to the blastocyst stage after parthenogenetic activation in comparison with oocytes that did not received MIT or were injected with solution (27.3% vs 17.4%，7.84%，P<0.05)，however，there were no significant difference in the blastocyst rates of the third level of oocytes than the control group and the nothing injection group after MIT.The ΔΨm of buffalo preimplantation embryo presented an increased trend during embryonic development，and the ΔΨm of embryo derived from MIT group was significantly higher than that of the control group (P<0.05).These results showed that the mtDNA copy numbers in different quality buffalo oocytes have significant differences，and the mtDNA copy numbers of buffalo oocytes are positively related with the quality of oocytes and their developmental potential，and the developmental ability of the second level of oocytes can be improve by MIT.

Key words: mitochondrial transplantation, mtDNA copy number, mitochondrial membrane potential, buffalo, follicular granulosa cell

CLC Number:

GAO Ya-ke，LU Feng-hua，WU Zhu-lian，MA Fan，LIU Xiao-hua，DU Shan-shan，SHI De-shun. Effects of Mitochondrial Transplant on the Developmental Potential of Buffalo Oocytes[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 583-591.

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Effects of Mitochondrial Transplant on the Developmental Potential of Buffalo Oocytes

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