鸳鸯鸭恒定链两异构体组织转录模式研究

doi:10.11843/j.issn.0366-6964.2014.12.018

Abstract

Abstract:

Invariant chain (Ii)，as a chaperone of MHC Ⅱ，plays an important role in antigen presentation by promoting MHC Ⅱ to maturate，capture antigen and guide antigen presentation in vertebrates.Ii isoforms containing a thyroglobulin-like domain (Tg domain) are also expressed by a single Ii gene through alternative splicing transcription.Ii isoforms with Tg domain involve in regulating the antigen presentation of MHC class Ⅱ molecule.The research on function and mechanism of Ii isoforms is more in-depth in mammals.But the main form，expression level and the functional relationship of Ii isoforms in tissues of birds including muscovy duck (Cairina moschata) are not fully clear.The Ii of muscovy duck (MDIi) has two isoforms.To explore the tissue expression patterns of two Ii isoforms of Muscovy duck，the real-time relative quantitative PCR (RQ-PCR) assay was established to detect the transcription levels of MDIi-1 and MDIi-2 and the transcription characteristics of MDIi-1 and MDIi-2 were compared in variant tissues or organs.Results showed that the lowest transcription level of MDIi-1 was shown in bursa fundus，therefore used as calibrator.Compared with calibrator，MDIi-1 was found at the highest transcription levels in spleen and intestinal mucosa，and the secondary highest ones in bursa stipe，liver，kidney，thymus，and blood.The rest organs transcribed MDIi-1 at very low levels.For MDIi-2, spleen，bursa stipe，and intestinal mucosa transcribed MDIi-2 at highest levels over the calibrator.The MDIi-2 transcription levels in thymus，lung，and kidney were slightly lower than the calibrator.The MDIi-2 transcription levels of other tissues were far below the calibrator and that of bursa fundus also was the lowest one.But MDIi-1 was transcribed markedly higher than MDIi-2 in the same tissue or organs.However, the transcription of MDIi-1 has a high correlation with the transcription of MDIi-2 in all tested tissues or organs.The correlation coefficient is 0.850 7.Thus two invariant chain isoforms, MDIi-1 and MDIi-2，were co-transcribed in all detected tissues or organs of Muscovy duck，while MDIi-1 was the main form of MDIi isorforms in each tissue or organs.MDIi-1 and MDIi-2 were all high transcribed in the tissues or organs with immune defense functions among detected tissues.The differential transcription patterns of MDIi-1 and MDIi-2 in different tissues or organs of muscovy duck may provide the base for further research on MDIi function mechanism and gene vaccine based on MDIi in the future.

Key words: muscovy duck, invariant chain, isoform, real-time relative quantitative PCR, specific transcription

CLC Number:

S852.4

LIU Sheng-jie,WU Chao,NI Qing-sheng,YU Wei-yi . Research on Tissue Transcription Patterns of Two Invariant Chain Isoforms of Muscovy Duck (Cairina moschata)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2034-2042.

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Research on Tissue Transcription Patterns of Two Invariant Chain Isoforms of Muscovy Duck (Cairina moschata)

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