Abstract:

The purpose of this study was to develop a novel aqueous or oil-in-water adjuvant for the Mycoplasma hyopneumoniae inactivated vaccine as well as to reduce the side effect of the vaccine.The Kunming mice were used as the animal model to evaluate the immune enhancement effect of different adjuvants to the inactivated vaccine.After immunization，the response of lymphocyte proliferation and the production of serum antibody IgG were evaluated.The experiments were carried out as two individual parts and 11 kinds of adjuvants were tested in total.The results indicated that the carbomer ₉₇₄ + ISCOM-matrix mixture adjuvant and the chitosan + levamisole mixture adjuvant strongly induced high levels of cellular immune and humoral immune response，better than the effect of the carbomer ₉₇₄ + levamisole mixture adjuvant，the carbomer ₉₇₄ + levamisole+ astragalus polysaccharides mixture adjuvant and the ISA 11R VG adjuvant.Animals of the groups of the beta- glucan+ levamisole mixture adjuvant，the DEAE-dextran+ levamisole+ astragalus polysaccharide mixture adjuvant and the beta- glucan+ levamisole+ astragalus polysaccharide mixture adjuvant had obvious cellular immune response，but weak humoral immune response.Additionally，animals of the group of the GEL 01 ST adjuvant had obvious humoral immune response，but weak cellular immune response.The carbomer ₉₇₄ +ISCOM-matrix mixture adjuvant and the chitosan + levamisole mixture adjuvant had the best effect on both cellular and humoral immune response.This data would help in the subsequent research on the inactivated vaccine against Mycoplasma hyopneumoniae.

Key words: acquous adjuvant, oil-in-water adjuvants, Mycoplasma hyopneumoniae inactivated vaccine, cellular immune response, humoral immune response