氟对小鼠脾淋巴细胞ERK通路相关基因及蛋白质表达的影响

doi:10.11843/j.issn.0366-6964.2014.11.021

Abstract

Abstract:

The current study was undertaken to study the effects of fluoride on the expression of ERK signaling pathway associated gene and proteins in the splenic lymphocytes of mouse.The splenic lymphocytes of the different level of fluoride-treated mouse in vivo and the splenic lymphocytes with the different level of sodium fluoride in vitro were cultured in RPMI-1640 medium with bacterial lipopolysaccharide，then the mRNA level of ras/c-raf/MEK1/ERK1 and the protein expression of ERK1 were detected using the Real-time PCR and Western blotting.The results showed that activation of the ERK pathway in mouse splenic lymphocytes can be inhibited by fluoride in low concentrations，but with increasing fluoride concentration，the degree of inhibition was reduced，ERK pathway activation was turn into promoting.These results suggested that fluoride can affect the activation of ERK pathway in mouse splenic lymphocytes，thus further influence the function of the immune system.

Key words: sodium fluoride, mouse splenic lymphocytes, ERK1, gene transcription, protein expression

CLC Number:

S859.81

XU Gang，WANG Jin-ming，MA Shu-hui，ZHAN Ya-qi，MA Hai-li. Effects of Fluoride on the Expression of ERK Signaling Pathway Associated Gene and Proteins in the Splenic Lymphocytes of Mouse[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1888-1894.

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Effects of Fluoride on the Expression of ERK Signaling Pathway Associated Gene and Proteins in the Splenic Lymphocytes of Mouse

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