猪流行性腹泻病毒S、N和ORF3基因的遗传变异分析

doi:10.11843/j.issn.0366-6964.2014.11.013

Abstract

Abstract:

To investigate the variation in structure protein of porcine epidemic diarrhea virus (PEDV)，S，N and ORF3 genes of 7 PEDV strains were cloned and sequenced from different farms in Fujian in 2012-2013. The results indicated that the nucleotide sequences of S gene shared 98.6%-99.4% identity within them，shared 93.7%-94.1% identity with attenuated DR13 strain，shared 93.8%-94.1% identity with CV777 strain. Compared with CV777 strain，there were 64 variant amino acids，and 3 amino acids different from other prevalent strains at home and abroad，and might be new variations，and 5 amino acids insertion，and 2 amino acids deletion. The amino acids insertion and deletion were similar to NPPED2008_2 strain isolated in Thailand. The nucleotide sequence of N and ORF3 genes shared 96.5%-99.7% and 99.7%-100% identity within them，and shared 96.6%-98.0% and 96.6%-96.9% identity with CV777 strain，respectively. Phylogenetic analysis indicated that S and ORF3 genes of the 7 PEDV strains had a closer relationship with the Thailand strain，and N gene had a closer relationship with the CV777 strain. The results showed that S and ORF3 genes of the 7 PEDV strains varied greatly，and these strains might origin from Thailand and Korea strains.

Key words: porcine epidemic diarrhea virus, S gene, N gene, ORF3 gene, genetic variation analysis

CLC Number:

S852.659.6

WANG Long-bai，LIN Yu-sheng，CHE Yong-liang，WU Xue-min，CHEN Ru-jing，SHAO Liang-ping，ZHOU Lun-jiang. Genetic Variation Analysis of S，N and ORF3 Genes of Porcine Epidemic Diarrhea Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1830-1836.

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Genetic Variation Analysis of S，N and ORF3 Genes of Porcine Epidemic Diarrhea Virus

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