安徽地方猪TLR4基因第3外显子的SNP分析

doi:10.11843/j.issn.0366-6964.2014.11.005

Abstract

Abstract:

In this study，using the PCR-SSCP method，the genetic variations in exon 3 of TLR4 were detected among 5 pig populations including Wannan Black，Wei，Anqing Liu White，Huoshou Black and Landrace pigs，the total individual number was 354.This study was conducted to analyze the polymorphisms of exon 3 of TLR4 in Anhui native pig breeds and aimed to provide a theoretical foundation for further research on the role of TLR4 in immune and defense system.Two SNPs were found，including G417A and C1027A.G417A was a synonymous SNP site，2 genotypes (GG，GA) were detected only in Wannan Black，Wei and Anqing Liu White pigs，the 3 populations were all in low polymorphism situation，and in Hardy-Weinberg equilibrium (P>0.05).C1027A was a nonsynonymous SNP site and changed the character of amino acid，3 genotypes (CC,CA，AA) were detected in Wannan Black，Wei，Anqing Liu White，Huoshou Black and Landrace pigs，the 5 populations were all in average polymorphism situation，and in Hardy-Weinberg equilibrium (P>0.05).The χ² test results of independence for the genotypes distribution of each SNP locus showed that the genotype frequencies for Landrace and Wannan Black，Wei，Anqing Liu White，Huoshou Black pigs were significantly different (P<0.01) at G417A and C1027A loci.These results indicated that the exon 3 sequences of TLR4 were relatively conservative in Anhui native pig breeds，and these populations had low polymorphism and genetic variation.In addition，the association between the differential genotypes distribution of C1027A locus in multiple pigs and the different disease resistance capability for pig breeds was worthy of further research.

Key words: Anhui native pig breeds, TLR4 gene, SNP, population genetics analysis

CLC Number:

S828
S813.3

DING Yue-yun，ZHU Wei-hua，XUE Wei-wei，MENG Yun，HUANG Long，ZHANG Xiao-dong，YIN Zong-jun. Single Nucleotide Polymorphism (SNP) Analysis of TLR4 Gene Exon 3 in Anhui Native Pig Breeds[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1767-1774.

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Single Nucleotide Polymorphism (SNP) Analysis of TLR4 Gene Exon 3 in Anhui Native Pig Breeds

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