脂多糖对母兔下丘脑、卵巢、输卵管中TLR4及其相关细胞因子表达的影响

doi:10.11843/j.issn.0366-6964.2014.06.008

Abstract

Abstract:

The aim of this study was to reveal the effect of LPS on expression level of TLR4，and downstream inflammatory cytokines，IL-1β and IL-6 in hypothalamus，oviduct and ovary.36 healthy New Zealand female rabbits，4 animals were non-treatment as 0.0 h，16 animals were injected with LPS according to the dosage of 0.5 mg•kg^-1 body weight as LPS group，and other 16 ones were injected with saline (LPS carrier) as control group.The hypothalamus，ovary and oviduct were collected at 0.0，1.5 ，3.0，6.0 and 12.0 h after injection.The expression level of TLR4，IL-1β and IL-6 were analyzed by real-time PCR and the concentration of IL-1β and IL-6 were examined by ELISA.The results showed：TLR4 was expressed in all experimental tissue samples，but LPS induced the significant up-regulation of TLR4 mRNA only in the ovary (P<0.05).And LPS significantly induced the expression level of IL-1β and IL-6 in the hypothalamus，ovary and oviduct (P<0.05)，but the immunosuppressant of LPS had obviously different in three organs.Namely，immunoresponse in the hypothalamus response to LPS was more sensitive and the duration of immunoresponse was longer than in the ovary and oviduct.According to the results we speculate ：LPS immune stress can increase TLR4，IL-1β，IL-6 expression in the hypothalamus and also increase expression of IL-1β，IL-6 in the ovaries，and oviduct.

Key words: lipopolysaccharide (LPS), TLR4, immune cytokines, oviduct, ovary, hypothalamus

CLC Number:

HE Zi-biao，LAN Rui-xia，CHEN Chen，LIU Shu-jun，SHI Yi，CHEN Shi-yi，LAI Song-jia，ZHANG Ming. Effect of LPS on the Expression of TLR4 and Related Cytokines in Oviduct，Ovary and Hypothalamus of Rabbits[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 910-917.

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Effect of LPS on the Expression of TLR4 and Related Cytokines in Oviduct，Ovary and Hypothalamus of Rabbits

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