ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (3): 353-357.

• 遗传繁育 • Previous Articles     Next Articles

Expression of Porcine Globular Adiponectin Gene in Lactococcus lactis

LIU Aisha1，LI Yan2*，HU Wenfeng1*，WU Tongshan2， LI Jiaqi3,
CHEN Zhenwei1，JIANG Guanyao1，LI Li1   

  1. 1. Laboratory of Using Microbial, Department of Bioengineering, College of Food Science, South China Agricultural University, Guangzhou 510642，China； 2. Institute of Dongguan Animal Husbandry and Science, Dongguan 523086，China; 3. College of Animal Science, South China Agricultural University, Guangzhou 510640, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-03-28 Published:2012-03-28
  • Contact: LI Yan2*，HU Wenfeng1

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Abstract: This experiment was conducted to clone, express porcine globular Adiponectin (gAd) gene in recombinant Lactococcus lactis NZ9000 and analyze the biological activity of expression product. The total RNA was extracted from porcine adipose tissue, and the Adiponectin gene was cloned into T plasmid for gene sequencing. Primers containing the corresponding restriction digestion sites and Histag sequence were designed, and then gAd gene was subcloned into expression vector pNZ8048 and expressed in recombinant L. lactis NZ9000. After purification, the immunological acitivity of recombinant protein was determined by Western blotting. To evaluate the biological activity of recombinant gAd, purified gAd was injected into the mice fed highglucose diet. The results showed that the gAd gene was successfully obtained and expressed in recombinant L. lactis NZ9000. The molecular weight of gAd protein is 17 ku. The recombinant gAd significantly decreased blood glucose of mice. This work established a good foundation for further study on the regulation of porcine lipometabolism by recombinant globular adiponectin.

Key words: porcine, adiponectin, globular domain, Lactococcus lactis