Abstract: This study was conducted to investigate the transcription change of chicken NLRC5 receptor and IFN genes initiated by different bacterial endotoxin, aiming to provide a theoretical basis on poultry NLRC5 receptor function and its regulation mechanism on innate immune. Different concentrations (0, 0.1, 1.0, 10.0 μg·mL-1) of endotoxin from Escherichia coli(E.coli,EC) and Salmonella enteritidis(S. enteriditis,SE) were used to stimulate the chicken macrophage cell line (HD11) for 2,4 and 6 hours, respectively. Total RNA was isolated from cells at different time points, then the transcription difference of NLRC5, IFNA and IFNB genes between different treatments were determined by quantitative realtime RTPCR, using SYBR Green RTPCR, and immune response for HD11 cells to different bacterial endotoxin were finally analyzed. In comparison with the control group, 1.0 and 10.0 μg·mL-1 of the ECLPS, SELPS could induce immune responses in HD11 cells, whereas 0.1 μg·mL-1 group failed to induce responses successfully. The immune response time induced by different bacterial LPS was different. NLRC5 and IFNB gene transcription induced by ECLPS, SELPS showed upregulation and reached the peak at 6 hours poststimulation, which presented significant difference with that of 2,4 hours poststimulation, but IFNA gene transcription induced by ECLPS reached the peak at 2,6 hours, while was down regulated at 4 hours poststimulation, showing as "V"type transcription trend. The transcription of all three genes induced by SELPS reached the peak at 6 hours poststimulation and presented significant difference with that of 2, 4 hours and control group, showing as one up trend line with time going on. The results showed that the IFNA, IFNB and NLRC5 receptors participated jointly the immune response to bacterial endotoxins, and NLRC5 genes may have regulate function on innate immune in chicken.

Key words: NLRC5, interferon, chicken, innate immune, regulation