Abstract:

The aim of the study is to clone the fulllength coding region of the porcine PNLIPRP2 (pancreatic lipaserelated protein 2) gene, analyze the molecular characteristic, and investigate its expression in the major tissues. The CDS of porcine PNLIPRP2 gene was cloned using the in Silico Cloning and RTPCR methods. The sequence was submitted to the GenBank. Many softwares (e.g., GENSCAN) and online resources (e.g., NCBI) were used for the analysis of bioinformatics and comparative genomics. The expression of PNLIPRP2 gene was investigated by RTPCR in 12 different tissues of 2monthold Large White pig. The fulllength CDS of the porcine PNLIPRP2 is 1 416 bp (EU715062) encoding a protein of 471 amino acids which contains a conserved PLAT domain of pancreatic triglyceride lipase (PLATPL). Porcine PNLIPRP2 is evolutionary more near to human and chimpanzee than other species. Further bioinformatics analysis showed that porcine PNLIPRP2 gene was located on porcine chromosome 14 and contained 12 exons and 11 introns. In addition, porcine PNLIPRP2 mRNA was the most abundant in intestine, followed by the mordrate level in brain and fat, the lowest level in bone marrow and stomach, and almost no expression in other tissues. The CDS fulllength of the porcine PNLIPRP2 gene was cloned. The molecular characteristic and its expression patterns in different tissues were also described. The results indicated that porcine PNLIPRP2 gene may be an important candidate gene in regarding to the fatty deposition and the immunity.

Key words: pig, PNLIPRP2 gene, sequence analysis, semiquantitative RTPCR