ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2009, Vol. 40 ›› Issue (9): 1303-1307.

• 遗传繁育 • Previous Articles     Next Articles

Detection of the FecXR Mutation of BMP15 Gene in Sheep and Goats

DI Ran 1, FENG Tao 2, CHU Mingxing 1*, ZHANG Yingjie 2, FANG Li 1

  

  1. 1. Key Laboratory of Farm Animal Genetic Resources and Utilization of Ministry of Agriculture,Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
  • Received:2008-04-07 Revised:1900-01-01 Online:2009-09-24 Published:2009-09-24
  • Contact: CHU Mingxing

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Abstract:

To detect the mutations of bone morphogenetic protein 15(BMP15) gene in both high prolificacy breeds (Small Tail Han sheep, Hu sheep, Jining Grey goat and Boer goat) and low prolificacy breeds (Dorset sheep, Texel sheep, Inner Mongolia Cashmere goat and Angora goat), the method of PCRSSCP was used. Both the nucleotide sequences and the amino acid sequences were compared in the amplification fragments of both Small Tail Han sheep and Jining Grey goat. The results indicated that none of the four sheep and the four goat breeds carried the same FecXR mutation of the BMP15 gene as Rasa Aragonesa sheep. The nucleotide sequence of Small Tail Han sheep was completely identical with that of the sheep BMP15 sequence (AF236079, NM_001114767) in the GenBank. Three base substitutes (T529G, C530G and T576C) and two amino acid changes (V155G and S171P) were found in Jining Grey goat compared with Small Tail Han sheep. The FecXR mutation of the BMP15 gene had no significant effect on high prolificacy of Small Tail Han sheep, Hu sheep, Jining Grey goat and Boer goat.

Key words: sheep, goat, prolificacy, BMP15 gene, FecXR mutation, PCRSSCP