Abstract: The resistant phenotypes and the genotypes of extendedspectrum βlactamases were detected in Salmonella pullorum by microdilution method and PCRsequencing. The results indicated that the isolates were high resistance to ampicillin, thirdgeneration cephalosporins, florfenicol, and enrofloxacin, although sensitive to cefepime, ceftiofur/sulbactam (2∶1), and cefoperazone/tazobactam (4∶1). Eight isolates harboured five kinds of ESBL genotypes (six TEM1, three CTXM65, one CTXM90, two OXA1 and one OXA10). To our knowledge, this study is the first to describe and name the CTXM90. The amino acid sequence of CTXM90 contains only one amino acid alteration, when contrasting to that of CTXM14 or CTXM65 (80Ala→Val, and 275Arg→Ser, respectively). Based on amino acid sequence similarities, CTXM90 is classified in the CTXM9 subgroup. The results illustrated that eight Salmonella pullorum showed serious multiresistance and carried complex extendedspectrum βlactamase genes.