Abstract: To investigate the prevalence of plasmidmediated quinolone resistance (PMQR) in Salmonella isolates from food animals, susceptibility of 316 Salmonella isolates from food animals to 20 antimicrobial agents were determined by the broth microdilution method, the prevalence of PMQR genes in the isolates were detected by PCR. The results showed that 316 Salmonella isolates exhibited different resistance to 20 antimicrobial agents, 95.57% of isolates showed multiresistance; The positive rates of qnrA, qnrC, qnrD, qnrS, qepA, qnrB, aac(6′)Ibcr, oqxA and oqxB genes were 0%, 0%, 0%, 0%, 0%, 7.91%, 15.19%, 7.91% and 8.86%, respectively. oqxAB gene was the first time reported in Salmonella; 98.11% of PMQRpositive strains, which showed multipleresistance to 817 antimicrobials, harboured two or more resistant genes, qnrB & aac(6′)Ibcr was the most common in these strains; The results of PFGE displayed 53 PMQRpositive strains belonged to 5 PFGE patterns. Some strains ,which had different resistant phenotype or genotype, showed same PFGE patterns. In conclusion, 316 Salmonella isolates tested showed serious resistance to antimicrobial agents; qnrB, aac(6′)Ibcr and oqxAB genes were frequence in strains; Clonal strain was epidemic in some resistant isolates from different origins.