ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (5): 779-784.doi:

• 预防兽医 • Previous Articles     Next Articles

Development and Application of Microsatelliteprimered PCR for Rapidly Identifying Dermatophytes from Rabbits

LI Daijun1, ZHOU Yufa2, LIU Jingbo1, GAO Lili1, ZHAO Xiaonan1,
CAI Yumei1*, CHAI Tongjie1*   

  1. 1. College of Animal Sciences, Shandong Agricultural University, Tai’an 271000, China;2. Animal Husbandry Bureau of Daiyue Region, Tai’an 271000, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28
  • Contact: CAI Yumei1*, CHAI Tongjie1*

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Abstract: The study was carried out to establish an accurate and rapid method to identify dermatophytes from rabbits by microsatelliteprimered polymerase chain reaction (PCR) and NTSYSpc2.10 software. The DNAs of Trichophyton mentagrophyton, Microsporum gypseum and Microsporum canis were amplified by the primer (GACA)4, and then their DNA fingerprint polymorphism was analyzed using NTSYSpc2.10 software. Based on the above results, the identification method was confirmed. Finally, a total of 21 clinical isolates identified by morphology were identified by the method. The results showed that: 1) The three kinds of rabbit dermatophytes have obviously different DNA fingerprints; 2) The rabbit dermatophytes of the same kind have high genetic similarity (90%100%); 3) The identified results of clinical isolates by this method were consistent with the morphological identification results. The results indicated that dermatophytes from rabbits could be rapidly and accurately identified by the microsatelliteprimered PCR in combination with NTSYSpc2.10 software.